<div class="csl-bib-body">
<div class="csl-entry">Rajamanickam, V., Metzger, K., Schmid, C., & Spadiut, O. (2017). A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris. <i>Microbial Cell Factories</i>, <i>16</i>, 1–7. https://doi.org/10.1186/s12934-017-0768-8</div>
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dc.identifier.issn
1475-2859
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/19884
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dc.description.abstract
The methylotrophic yeast Pichia pastoris is a well-studied host organism for recombinant protein production, which is usually regulated either by a constitutive promoter (e.g. promoter of glyceraldehyde-3-phosphate dehydrogenase; PGAP) or an inducible promoter (e.g. promoter of alcohol oxidase 1; PAOX1). Both promoter systems have several advantages and disadvantages; with one of the main disadvantages being their lack of tunability. Various novel promoter systems, which are either inducible or de-repressed, allowing higher degrees of freedom, have been reported. Recently, bi-directional promoter systems in P. pastoris with two promoter systems regulating recombinant expression of one or more genes were developed. In this study, we introduce a novel bi-directional promoter system combining a modified catalase promoter system (PDC; derepressible and inducible) and the traditional PAOX1, allowing tunable recombinant protein production.
en
dc.language.iso
en
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dc.publisher
BMC
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dc.relation.ispartof
Microbial Cell Factories
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Bi-directional promoter
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dc.subject
Bioprocess Development
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dc.subject
mixed feed
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dc.subject
Pichia pastoris
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dc.subject
Tunable recombinant protein production
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dc.title
A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris