<div class="csl-bib-body">
<div class="csl-entry">van Nieuwenhoven, R., Gebeshuber, I.-C., & Sutterluety, C. (2024, June 10). <i>Redefining 3D Bioprinting: Vertical Bio-Ink Deposition for Improved Biofabrication</i> [Poster Presentation]. Living Maschines 2024, Chicago, United States of America (the).</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/199534
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dc.description.abstract
A novel approach to Extrusion-based 3D printing named Vertical Pillar Extrusion (VPE) is proposed, wherein living cells are deposited vertically into a supportive substrate, diverging from the established horizontal deposition method. Traditional substrate-based 3D bio-printing methodologies primarily employ horizontal layering techniques. This innovative, low-cost technique entails the utilisation of a needle-equipped nozzle, minimising substrate disturbance by abstaining from horizontal movements within the medium. Bio-Ink injection occurs exclusively from the top, while extrusion is initiated upon needle retraction from the substrate. Extrusion during retraction mitigates the sheer stresses for the living component of the bio-inks. Various bio-ink nozzle types (tapered and straight) will be analysed to identify the optimal nozzle configuration that maximises the survival rate of the living components within the bio-ink. We aim to utilise this printing technique for bio-inks derived from algae and bacteria and for printing a bio-ink containing dissolved chitosan onto a substrate with an acetylating agent to fabricate 3D-printed chitin structures.
The efficacy of this 3D printing technique is analysed via Computational Fluid Dynamics (CFD) simulations to predict the behaviour and interaction of the bio-ink with the substrate. Experimental validation of the results will be conducted to ensure the accuracy and reliability of the technique's practical applicability.
The proposed methodology involves coding a new g-code generator, predicated on Digital Light Processing (DLP) slicer shutter images dictating the voxel locations for material deposition. We anticipate that this pioneering 3D printing technique will yield substantial enhancements in the precision of bio-ink deposition for the fabrication of 3D-printed engineered living materials.
en
dc.language.iso
en
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dc.subject
ELM
en
dc.subject
Vertical Pillar Extrusion
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dc.subject
3D bio-ink printing
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dc.title
Redefining 3D Bioprinting: Vertical Bio-Ink Deposition for Improved Biofabrication
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Poster Presentation
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tuw.researchTopic.id
M2
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E134-03 - Forschungsbereich Atomic and Plasma Physics
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tuw.author.orcid
0000-0001-8879-2302
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tuw.event.name
Living Maschines 2024
en
tuw.event.startdate
08-06-2024
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tuw.event.enddate
11-07-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Chicago
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tuw.event.country
US
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tuw.event.presenter
van Nieuwenhoven, Richard
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wb.sciencebranch
Biologie
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wb.sciencebranch
Physik, Astronomie
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
1030
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wb.sciencebranch.value
50
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wb.sciencebranch.value
50
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E134-03 - Forschungsbereich Atomic and Plasma Physics
