<div class="csl-bib-body">
<div class="csl-entry">Eilenberger, C., Rothbauer, M., Ehmoser, E.-K., Ertl, P., & Küpcü, S. (2019). Effect of Spheroidal Age on Sorafenib Diffusivity and Toxicity in a 3D HepG2 Spheroid Model. <i>Scientific Reports</i>, <i>9</i>, 1–11. https://doi.org/10.1038/s41598-019-41273-3</div>
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dc.identifier.issn
2045-2322
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/19982
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dc.description.abstract
The enhanced predictive power of 3D multi-cellular spheroids in comparison to conventional monolayer cultures makes them a promising drug screening tool. However, clinical translation for pharmacology and toxicology is lagging its technological progression. Even though spheroids show a biological complexity resembling native tissue, standardization and validation of drug screening protocols are influenced by continuously changing physiological parameters during spheroid formation. Such cellular heterogeneities impede the comparability of drug efficacy studies and toxicological screenings. In this paper, we demonstrated that aside from already well-established physiological parameters, spheroidal age is an additional critical parameter that impacts drug diffusivity and toxicity in 3D cell culture models. HepG2 spheroids were generated and maintained on a self-assembled ultra-low attachment nanobiointerface and characterized regarding time-dependent changes in morphology, functionality as well as anti-cancer drug resistance. We demonstrated that spheroidal aging directly influences drug response due to the evolution of spheroid micro-structure and organo-typic functions, that alter inward diffusion, thus drug uptake.
en
dc.language.iso
en
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dc.publisher
NATURE PORTFOLIO
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dc.relation.ispartof
Scientific Reports
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
biological techniques
en
dc.subject
tissue engineering
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dc.subject
Toxicology
en
dc.title
Effect of Spheroidal Age on Sorafenib Diffusivity and Toxicity in a 3D HepG2 Spheroid Model