<div class="csl-bib-body">
<div class="csl-entry">Pflügl, S., Hocq, R. V., Horvath, J. A., Stumptner, M., & Steiner, L. (2024, June 5). <i>Gas fermentation goes thermophilic: Thermoanaerobacter kivui as a promising host for acetogenic fermentation of syngas from biomass gasification</i> [Conference Presentation]. 20th International Conference on Renewable Resources and Biorefineries, Brüssel, Belgium.</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/200070
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dc.description.abstract
Conversion of gaseous one carbon substrates (CO, CO2) and H2 by acetogenic bacteria is a promising technology to establish sustainable bioproduction scenarios. The thermophilic acetogen Thermoanaerobacter kivui (Topt = 66 °C) grows on H2/CO2 in chemically defined mineral medium with growth rates (doubling time: 2 h), exceeding those of mesophilic acetogens. Nevertheless, there is currently no bioprocessing system available for quantitative characterization of T. kivui as a model thermophilic acetogen under well-defined bioreactor conditions. In this study, we established a continuous high-temperature gas fermentation system and characterized T. kivui wildtype as well as a strain adapted to grow on CO (referred to as CO-1). T. kivui CO-1 grew in a 100 % CO gas phase in chemically defined mineral medium with a growth rate of up to 0.25 h-1 (doubling time: 2.8 h) and adaptation occurred in as little as 30 generations, similar to previous work by Weghoff and Müller (2016) using rich medium [1]. To characterize the physiology of strain CO-1 in more detail, steady state chemostat cultures operated at specific growth rates of 0.10-0.20 h-1 were used to quantify growth, gas consumption, and acetate production on H2/CO2, syngas and pure CO. Genomic analysis of the clonal strain CO-1 using short and long read sequencing technologies indicated that a handful of SNPs and large-scale genomic rearrangements might be responsible for the successful adaptation of T. kivui to CO. Transcriptomic analysis gave further clues how the physiology of strain CO-1 is adapted to growth on CO. Next, the use of syngas obtained via steam gasification of softwood pellets as a feedstock for gas fermentation with T. kivui was evaluated. To that end, we evaluated (i) the impact of potential inhibitors generated during biomass gasification (aromatic compounds BTEX, H2S and cyanide) and (ii) recovery of nitrogen from the syngas stream and utilization as nitrogen source for T. kivui to reduce the resource footprint of the fermentation process. Overall, synthesis gas generated from biomass gasification was successfully utilized for gas fermentation with T. kivui in continuous mode in 200 mL stirred tank bioreactors as well as a 20 L bubble column bioreactor. Collectively, the results of this study represent a first step toward establishing high-temperature gas fermentation processes.
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dc.description.sponsorship
Christian Doppler Forschungsgesells
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dc.language.iso
en
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dc.subject
Gas fermentation
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dc.subject
Acetogens
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dc.subject
Circular carbon economy
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dc.subject
Industrial biotechnology
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dc.subject
biomass valorization
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dc.title
Gas fermentation goes thermophilic: Thermoanaerobacter kivui as a promising host for acetogenic fermentation of syngas from biomass gasification
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
CAZy
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dc.type.category
Conference Presentation
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tuw.project.title
Christian Doppler Labor für optimierte Expression von Kohlenhydrat-aktiven Enzymen
