reposiTUm: Gas fermentation goes thermophilic: Thermoanaerobacter kivui as a promising host for acetogenic fermentation of syngas from biomass gasification

DC Element

Wert

Sprache

dc.contributor.author

Steiner, Lena

dc.contributor.author

Horvath, Josef Alexander

dc.contributor.author

Stumptner, Maja

dc.contributor.author

Reichebner, J. A.

dc.contributor.author

Hocq, Remi Vincent

dc.contributor.author

Benedikt, Florian

dc.contributor.author

Bartik, Alexander

dc.contributor.author

Müller, Stefan

dc.contributor.author

Pflügl, Stefan

dc.date.accessioned

2024-08-30T15:11:13Z

dc.date.available

2024-08-30T15:11:13Z

dc.date.issued

2024-08-14

dc.identifier.citation

<div class="csl-bib-body"> <div class="csl-entry">Steiner, L., Horvath, J. A., Stumptner, M., Reichebner, J. A., Hocq, R. V., Benedikt, F., Bartik, A., Müller, S., & Pflügl, S. (2024, August 14). <i>Gas fermentation goes thermophilic: Thermoanaerobacter kivui as a promising host for acetogenic fermentation of syngas from biomass gasification</i> [Poster Presentation]. Molecular Basis of Microbial One-Carbon Metabolism, Waterville Valley, United States of America (the). http://hdl.handle.net/20.500.12708/200072</div> </div>

dc.identifier.uri

http://hdl.handle.net/20.500.12708/200072

dc.description.abstract

Conversion of gaseous substrates (CO, CO2, H2) by acetogenic bacteria is a promising technology to establish sustainable bioproduction scenarios. The thermophilic acetogen Thermoanaerobacter kivui (Topt = 66 °C) grows on H2/CO2 in chemically defined mineral medium with high growth rates (doubling time: 2 h). However, there is currently no bioprocessing system available for quantitative characterization of T. kivui as a model thermophilic acetogen under well-defined bioreactor conditions. In this study, we established a continuous high-temperature gas fermentation system and characterized T. kivui wildtype as well as a strain with the ability to grow on CO (referred to as CO-1). T. kivui CO-1 grew in a 100 % CO gas phase in chemically defined mineral medium with a growth rate of up to 0.25 h-1 (doubling time: 2.8 h). To characterize the physiology of strain CO-1 in more detail, steady state chemostat cultures operated at specific growth rates of 0.10-0.20 h-1 were used to quantify growth, gas consumption, and acetate production on H2/CO2, syngas and pure CO. Transcriptomic analysis revealed that the gene expression levels of the energy converting hydrogenase 2 complex (Ech2) involved in energy conservation were increased. Next, the use of syngas obtained via steam gasification of softwood pellets as a feedstock for gas fermentation with T. kivui was evaluated. To that end, we evaluated (i) the impact of potential inhibitors generated during biomass gasification (aromatic compounds BTEX, H2S and cyanide) and (ii) recovery of nitrogen from the syngas stream and utilization as nitrogen source for T. kivui to reduce the resource footprint of the fermentation process. Overall, synthesis gas generated from biomass gasification was successfully utilized for gas fermentation with T. kivui in continuous mode in 200 mL stirred tank bioreactors as well as a 20 L bubble column bioreactor. Collectively, the results of this study represent a first step toward establishing high-temperature gas fermentation processes.

dc.description.sponsorship

Christian Doppler Forschungsgesells

dc.language.iso

dc.subject

Gas fermentation

dc.subject

Acetogens

dc.subject

Circular carbon economy

dc.subject

Industrial biotechnology

dc.subject

Biomass valorization

dc.title

Gas fermentation goes thermophilic: Thermoanaerobacter kivui as a promising host for acetogenic fermentation of syngas from biomass gasification

dc.type

Presentation

dc.type

Vortrag

dc.contributor.affiliation

TU Wien, Austria

dc.relation.grantno

CAZy

dc.type.category

Poster Presentation

tuw.project.title

Christian Doppler Labor für optimierte Expression von Kohlenhydrat-aktiven Enzymen

tuw.researchTopic.id

tuw.researchTopic.name

Biological and Bioactive Materials

tuw.researchTopic.name

Sustainable Production and Technologies

tuw.researchTopic.value

tuw.linking

https://www.grc.org/molecular-basis-of-microbial-one-carbon-metabolism-conference/2024/

tuw.publication.orgunit

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

tuw.publication.orgunit

E166-07-2 - Forschungsgruppe Industrieanlagendesign und Anwendung digitaler Methoden

tuw.author.orcid

0000-0002-0675-7376

tuw.author.orcid

0000-0002-8600-1375

tuw.author.orcid

0000-0001-9350-546X

tuw.author.orcid

0000-0001-8878-429X

tuw.author.orcid

0000-0001-8472-5073

tuw.event.name

Molecular Basis of Microbial One-Carbon Metabolism

tuw.event.startdate

11-08-2024

tuw.event.enddate

16-08-2024

tuw.event.online

On Site

tuw.event.type

Event for scientific audience

tuw.event.place

Waterville Valley

tuw.event.country

tuw.event.institution

Gordon Research Conference

tuw.event.presenter

Pflügl, Stefan

tuw.event.track

Single Track

wb.sciencebranch

Industrielle Biotechnologie

wb.sciencebranch.oefos

2090

wb.sciencebranch.value

100

item.cerifentitytype

Publications

item.languageiso639-1

item.fulltext

no Fulltext

item.openairetype

conference poster not in proceedings

item.openairecristype

http://purl.org/coar/resource_type/c_18co

item.grantfulltext

restricted

crisitem.project.funder

Christian Doppler Forschungsgesells

crisitem.project.grantno

CAZy

crisitem.author.dept

E166-07-2 - Forschungsgruppe Industrieanlagendesign und Anwendung digitaler Methoden

crisitem.author.dept

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

crisitem.author.dept

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

crisitem.author.dept

TU Wien

crisitem.author.dept

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

crisitem.author.dept

E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften

crisitem.author.dept

E166-07-2 - Forschungsgruppe Industrieanlagendesign und Anwendung digitaler Methoden

crisitem.author.dept

E166-07 - Forschungsbereich Brennstoff- und Energiesystemtechnik

crisitem.author.dept

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

crisitem.author.orcid

0000-0002-0675-7376

crisitem.author.orcid

0000-0002-8600-1375

crisitem.author.orcid

0000-0001-9350-546X

crisitem.author.orcid

0000-0001-8878-429X

crisitem.author.orcid

0000-0001-8472-5073

crisitem.author.parentorg

E166-07 - Forschungsbereich Brennstoff- und Energiesystemtechnik

crisitem.author.parentorg

E166-04 - Forschungsbereich Bioverfahrenstechnik

crisitem.author.parentorg

E166-04 - Forschungsbereich Bioverfahrenstechnik

crisitem.author.parentorg

E166-04 - Forschungsbereich Bioverfahrenstechnik

crisitem.author.parentorg

E150 - Fakultät für Technische Chemie

crisitem.author.parentorg

E166-07 - Forschungsbereich Brennstoff- und Energiesystemtechnik

crisitem.author.parentorg

E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften

crisitem.author.parentorg

E166-04 - Forschungsbereich Bioverfahrenstechnik

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