<div class="csl-bib-body">
<div class="csl-entry">Khattab, A., Grabowski, R., Schmees, C., & Zeck, G. M. (2024, March 21). <i>Non-invasive Monitoring of 3D-Tumor Spheroids using Electrical Impedance Spectroscopy</i> [Conference Presentation]. 9th International Winterschool on Bioelectronics (BioEl2024 ), Kirchberg in Tirol, Austria. http://hdl.handle.net/20.500.12708/202533</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/202533
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dc.description.abstract
Electrical Impedance Spectroscopy (EIS) is a non-invasive technique that allows monitoring of cancer cells and cancer tissue. EIS may be performed by two electrode-technique (2-ET) where both electrodes are used for excitation and recording purposes, or by four electrode-technique (4-ET) where the two outer electrodes are used for current excitation and the two inner electrodes record the resulting signal.1 The amplitudes of the impedances at specific frequencies (1-10 KHz) are typically used to identify differences between adherent cells, tissues and bare electrodes. Here we successfully used both techniques to closely monitor the adhesion of tumor spheroids using EIS.
Three-Dimensional Tumor Spheroids (3D-TS, diameter 200–500 µm) made of human colon cancer cell line HCT 116 were cultured on a Micro Electrode Array (MEA; electrode spacing: 70 µm). EIS was performed at three time points (4h, 21h, 66h) after interfacing the 3D-TS to the MEA. Using the MEA electrode map, we visually selected pairs of electrodes that are covered with 3D-TS. The impedance magnitude and phase shift were analyzed using custom-written scripts (Python).
Both techniques (2-ET and 4-ET) allow for reliable, noninvasive identification of adherent spheroids. The identification was confirmed using optical microscopy. In 2-ET measurements, we obtained significantly higher impedance amplitude for electrodes underneath the tumor spheroids as compared to bare electrodes. In 4-ET measurements, the impedance amplitudes are reduced but well distinguishable from control measurements. The 2-ET facilitates the generation of a 2D heat map, which closely mimics the size of the TS and its position on the MEA.
In 2-ET, the impedances of the electrodes influence the measurement, and the measured signal corresponds to the series connection of the spheroid and electrodes. However, In 4-ET, the sensing electrodes don’t carry any current thanks to the high input impedance of the amplifier and prevent their effect on the measurement. Ongoing experiments are performed to test the effect of anticancer drugs, and results will be presented.
1. Bragos, R. et al.”, Four Versus Two-Electrode Measurement Strategies for Cell Growing and Differentiation Monitoring Using Electrical Impedance Spectroscopy,” IEEE EMBS. pp:2106-2109, 2006.
en
dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.subject
Microelectrode Array
en
dc.subject
Impedance Spectroscopy
en
dc.subject
Tumor Spheroids
en
dc.title
Non-invasive Monitoring of 3D-Tumor Spheroids using Electrical Impedance Spectroscopy
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Tübingen, Germany
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dc.contributor.affiliation
University of Tübingen, Germany
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dc.relation.grantno
FO999895365
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dc.type.category
Conference Presentation
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tuw.project.title
Bioelektronische Plattform für personalisierte Krebstherapie auf der Grundlage von 3D-gedruckten Sphäroiden und von Patienten-abgeleiteten Mikrotumoren
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E363 - Institut für Biomedizinische Elektronik
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tuw.author.orcid
0000-0003-3998-9883
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tuw.event.name
9th International Winterschool on Bioelectronics (BioEl2024 )
en
tuw.event.startdate
17-03-2024
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tuw.event.enddate
22-03-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Kirchberg in Tirol
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tuw.event.country
AT
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tuw.event.institution
Johannes Kepler University Linz
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tuw.event.presenter
Khattab, Ahmed
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tuw.event.track
Multi Track
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wb.sciencebranch
Medizintechnik
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wb.sciencebranch
Biologie
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wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch.oefos
2060
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2020
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wb.sciencebranch.value
10
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wb.sciencebranch.value
50
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wb.sciencebranch.value
40
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.dept
University of Tübingen
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crisitem.author.dept
University of Tübingen
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.orcid
0000-0003-3998-9883
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
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crisitem.project.funder
FFG - Österr. Forschungsförderungs- gesellschaft mbH
