<div class="csl-bib-body">
<div class="csl-entry">Pekarsky, A., Reininger, M., & Spadiut, O. (2021). The impact of technical failures on recombinant production of soluble proteins in Escherichia coli: a case study on process and protein robustness. <i>Bioprocess and Biosystems Engineering</i>, <i>44</i>(6), 1049–1061. https://doi.org/10.1007/s00449-021-02514-w</div>
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dc.identifier.issn
1615-7591
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/20339
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dc.description.abstract
Technical failures lead to deviations in process parameters that can exceed studied process boundaries. The impact on cell and target protein is often unknown. However, investigations on common technical failures might yield interesting insights into process and protein robustness. Recently, we published a study on the impact of technical failures on an inclusion body process that showed high robustness due to the inherent stability of IBs. In this follow-up study, we investigated the influence of technical failures during production of two soluble, cytosolic proteins in E. coli BL21(DE3). Cell physiology, productivity and protein quality were analyzed, after technical failures in aeration, substrate supply, temperature and pH control had been triggered. In most cases, cell physiology and productivity recovered during a subsequent regeneration phase. However, our results highlight that some technical failures lead to persistent deviations and affect the quality of purified protein.
en
dc.language.iso
en
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dc.publisher
SPRINGER
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dc.relation.ispartof
Bioprocess and Biosystems Engineering
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Bioreactor
en
dc.subject
Cytosolic protein
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dc.subject
Escherichia coli
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dc.subject
process deviation
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dc.subject
protein glycation
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dc.subject
robustness
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dc.title
The impact of technical failures on recombinant production of soluble proteins in Escherichia coli: a case study on process and protein robustness
