<div class="csl-bib-body">
<div class="csl-entry">Khattab, A., Grabowski, R., Önning, L., Schmees, C., & Zeck, G. M. (2024, September 27). <i>Bioimpedance Spectroscopy for Noninvasive Monitoring of Tumor Spheroids</i> [Conference Presentation]. 17th International Workshop on Impedance Spectroscopy, Chemnitz, Germany. http://hdl.handle.net/20.500.12708/206725</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/206725
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dc.description.abstract
Three-dimensional (3D) in-vitro cell culture is an appropriate tool that closely mimics the behavior of in-vivo tissues. It can replicate the cell's growth rate, metabolic activity, cell-cell interaction and cell-substrate interaction more accurately than the standard two-dimensional cell culture. In this project, we combined the benefits of Electrical Impedance Spectroscopy (EIS) as a noninvasive and label-free monitoring technique, with 3D Tumor Spheroid (3D-TS) on a Microelectrode Array (MEA) to develop a bioelectronic platform for preclinical drug development. 3D-TS of roughly 300 ìm size made of a human colon cancer cell line were cultured on planar MEA comprising 60 electrodes. Control and drug treatment experiments were conducted using EIS in the frequency range of 100 Hz-1MHz to observe the 3d-TS response over time. After interfacing the 3D-TS with the MEA, we obtained significantly higher impedance amplitudes for electrodes underneath the 3D-TS than for bare electrodes. During the drug treatment, EIS revealed a time-dependent reduction in the impedance relative to the control groups. In conclusion, our platform allows for reliable, noninvasive recording from inside of 3D-TS without disaggregating the cells. This achievement allows simple preclinical drug screening applications.
en
dc.language.iso
en
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dc.subject
bioimpedance spectroscopy
en
dc.subject
drug development
en
dc.subject
microelectrode array
en
dc.subject
tumor spheroids
en
dc.title
Bioimpedance Spectroscopy for Noninvasive Monitoring of Tumor Spheroids
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Tübingen, Germany
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dc.contributor.affiliation
University of Tübingen, Germany
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dc.contributor.affiliation
University of Tübingen, Germany
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E363 - Institut für Biomedizinische Elektronik
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tuw.author.orcid
0000-0003-3998-9883
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tuw.event.name
17th International Workshop on Impedance Spectroscopy
en
dc.description.sponsorshipexternal
Austrian Research Promotion Agency (FFG)
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dc.description.sponsorshipexternal
German Federal Ministry for Economic Affairs and Climate Action (BMWK)
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dc.relation.grantnoexternal
895365
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dc.relation.grantnoexternal
KK5046205AJ2
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tuw.event.startdate
24-09-2024
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tuw.event.enddate
27-09-2024
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tuw.event.online
Hybrid
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tuw.event.type
Event for scientific audience
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tuw.event.place
Chemnitz
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tuw.event.country
DE
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tuw.event.institution
TU Chemnitz
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tuw.event.presenter
Khattab, Ahmed
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tuw.event.track
Single Track
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wb.sciencebranch
Biologie
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wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch
Mathematik
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2020
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wb.sciencebranch.oefos
1010
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wb.sciencebranch.value
50
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wb.sciencebranch.value
30
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wb.sciencebranch.value
20
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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item.grantfulltext
restricted
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.dept
University of Tübingen
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crisitem.author.dept
University of Tübingen
-
crisitem.author.dept
University of Tübingen
-
crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.orcid
0000-0003-3998-9883
-
crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
