<div class="csl-bib-body">
<div class="csl-entry">Khattab, A., Grabowski, R., Schmees, C., & Zeck, G. M. (2024, June 6). <i>Non-invasive Monitoring of 3D-Tumor Spheroids using Electrical Impedance Spectroscopy</i> [Poster Presentation]. MESS24 - Microelectronic Systems Symposium, Wien, Austria. http://hdl.handle.net/20.500.12708/206728</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/206728
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dc.description.abstract
Conventional in-vitro monitoring of cancer tissues is time-consuming and has limited accessibility. To enable a non-invasive technique to observe tumor behavior, Electrical Impedance Spectroscopy (EIS) can be employed. EIS may be performed by Two Electrode-Technique (2-ET), where both electrodes are used for excitation and recording purposes, or by Four Electrode-Technique (4-ET), where the two outer electrodes are used for current excitation, and the two inner electrodes record the resulting signal.1 Here, we successfully used both techniques to monitor the tumor adhesion and evaluate therapeutic interventions.
Three-dimensional Tumor Spheroids (3D-TS; diameter ~300 µm) made of human colon cancer cells were cultured on a Microelectrode Array (MEA; pitch: 70 µm). Control and drug treatment experiments (Ncontrol =14, Ndrug =10) were conducted using EIS to observe 3D-TS changes. Measurements were performed at 4h, 21h, and 66h after interfacing the 3D-TS with the MEA.
EIS allows for reliable, noninvasive identification of adherent spheroids. The identification was confirmed using optical microscopy. With both techniques (2-ET and 4-ET), we obtained significantly higher impedance amplitudes for electrodes underneath the tumor spheroids than for bare electrodes. During the drug treatment, EIS revealed a decrease in the impedance over time compared to the control groups. In the future, we plan to interface and record spheroids on flexible MEA.
1. Bragos, R. et al.”, Four Versus Two-Electrode Measurement Strategies for Cell Growing and Differentiation Monitoring Using Electrical Impedance Spectroscopy,” IEEE EMBS. pp:2106-2109, 2006.
en
dc.language.iso
en
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dc.subject
Impedance Spectroscopy
en
dc.subject
Microelectrodes array
en
dc.title
Non-invasive Monitoring of 3D-Tumor Spheroids using Electrical Impedance Spectroscopy
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Tübingen, Germany
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dc.contributor.affiliation
University of Tübingen, Germany
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dc.type.category
Poster Presentation
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E363 - Institut für Biomedizinische Elektronik
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tuw.author.orcid
0000-0003-3998-9883
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tuw.event.name
MESS24 - Microelectronic Systems Symposium
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dc.description.sponsorshipexternal
Austrian Research Promotion Agency (FFG)
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dc.description.sponsorshipexternal
German Federal Ministry for Economic Affairs and Climate Action (BMWK)
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dc.relation.grantnoexternal
895365
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dc.relation.grantnoexternal
KK5046205AJ2
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tuw.event.startdate
06-06-2024
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tuw.event.enddate
07-06-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
OVE Österreichischer Verband für Elektrotechnik
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tuw.event.presenter
Khattab, Ahmed
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tuw.event.track
Single Track
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wb.sciencebranch
Biologie
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wb.sciencebranch
Physik, Astronomie
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wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
1030
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wb.sciencebranch.oefos
2020
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wb.sciencebranch.value
30
-
wb.sciencebranch.value
30
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wb.sciencebranch.value
40
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.dept
University of Tübingen
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crisitem.author.dept
University of Tübingen
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.orcid
0000-0003-3998-9883
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
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crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
