<div class="csl-bib-body">
<div class="csl-entry">Soydan, M., & Conibear, A. C. (2024, May 29). <i>Impact of site-specific acetylation of HMGN1 on its interaction with damaged DNA</i> [Poster Presentation]. 2nd TCH Science Days: PhD & Postdoc Day 2024, Wien, Austria.</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/207453
-
dc.description.abstract
DNA and histone proteins form chromatin, with nucleosome core particles as the smallest unit. Chromatin can be altered by histone modification or DNA damage, which is caused by cellular metabolism or by external factors such as UV and ionizing radiation, or mutagenic chemicals. Many regulatory proteins are involved in chromatin function, including non-histone proteins like HMGN1, which is an intrinsically disordered, nucleosome binding nuclear protein. However, the effects of post-translational modifications on the biological function of HMGN1, such as DNA damage, remain largely unknown. We aim to investigate the effects of lysine acetylation in the nucleosome binding domain of HMGN1 on binding affinity to damaged DNA. In this poster, we present our approach to accessing site-specifically acetylated HMGN1 variants. We examined several semi-synthetic strategies and native chemical ligation sites for their synthetic accessibility and efficiency. The site-specifically acetylated HMGN1 variants will be used for electrophoretic mobility shift assays to determine how the modified protein–damaged DNA interactions change compared to native HMGN1. Understanding how acetylation of HMGN1 modulates binding to damaged DNA and its repair by PARP1 will provide insights into the role of HMGN1 in DNA repair and packaging.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
-
dc.language.iso
en
-
dc.subject
Protein chemistry
en
dc.title
Impact of site-specific acetylation of HMGN1 on its interaction with damaged DNA
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
P 36101-B
-
dc.type.category
Poster Presentation
-
tuw.project.title
Posttranslationale Modifikation von HMGN1 in DNA-Verpackung
-
tuw.researchTopic.id
M6
-
tuw.researchTopic.id
M8
-
tuw.researchTopic.name
Biological and Bioactive Materials
-
tuw.researchTopic.name
Structure-Property Relationsship
-
tuw.researchTopic.value
80
-
tuw.researchTopic.value
20
-
tuw.publication.orgunit
E163 - Institut für Angewandte Synthesechemie
-
tuw.author.orcid
0009-0008-5198-0025
-
tuw.author.orcid
0000-0002-5482-6225
-
tuw.event.name
2nd TCH Science Days: PhD & Postdoc Day 2024
en
tuw.event.startdate
29-05-2024
-
tuw.event.enddate
29-05-2024
-
tuw.event.online
On Site
-
tuw.event.type
Event for scientific audience
-
tuw.event.place
Wien
-
tuw.event.country
AT
-
tuw.event.institution
TU Wien
-
tuw.event.presenter
Soydan, Medine
-
wb.sciencebranch
Chemie
-
wb.sciencebranch
Chemische Verfahrenstechnik
-
wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
2040
-
wb.sciencebranch.oefos
3012
-
wb.sciencebranch.value
60
-
wb.sciencebranch.value
20
-
wb.sciencebranch.value
20
-
item.languageiso639-1
en
-
item.openairetype
conference poster not in proceedings
-
item.grantfulltext
restricted
-
item.fulltext
no Fulltext
-
item.cerifentitytype
Publications
-
item.openairecristype
http://purl.org/coar/resource_type/c_18co
-
crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
-
crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
-
crisitem.author.orcid
0009-0008-5198-0025
-
crisitem.author.orcid
0000-0002-5482-6225
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
