<div class="csl-bib-body">
<div class="csl-entry">Hoehlschen, J., Gosset, E., Tomin, T., & Birner-Grünberger, R. (2024, September 25). <i>Investigating the cardioprotective effects of SGLT-2 inhibitors</i> [Conference Presentation]. APMRS/CEEPC APMRS-CEEPC 2024, Wien, Austria. http://hdl.handle.net/20.500.12708/207566</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/207566
-
dc.description.abstract
Introduction
Beyond their initial approvement for treating diabetic conditions, Sodium-Glucose co-transporter 2 (SGLT-2) inhibitors have recently been found to also offer cardioprotective properties. Since cardiovascular problems are one of the major complications that diabetic patients face, this finding was groundbreaking. Meanwhile it was shown that the cardioprotective effects are not limited to diabetic patients and the inhibitors’ approvements have been expanded respectively. Observations from clinical studies hint in the direction that those effects exceed the glucose lowering effect without offering an explanation for the underlying mechanisms. Since the drug’s target protein SGLT-2 is exclusively expressed in the early proximal tubule where it is responsible for the reabsorption of glucose it remains inconclusive why the positive effects are observed in the heart.
Methods
To investigate what kind of an effect those drugs have on the proteome of cardiac cells, the human cardiac cell lines AC16 (cardiomyocytes derived from left ventricular heart tissue) and HCM (human cardiac myocytes) have been treated with Dapa-, Cana- and Empagliflozin in a physiological and a higher concentration. Besides classical proteomics also redox-proteomics and Glutathione as well as its dimer have been measured as marker for oxidative stress.
Results
Changes in Glutathione metabolism have been observed. This is among others reflected by changed ratios of reduced to oxidized Glutathione variants, as well as changes in abundance and oxidation state of proteins. Furthermore, proteomics data reveal upregulation of DNA replication and translation, additionally to changes in different mitochondria associated proteins.
Discussion
All drug treatments seem to strive to maintain oxidative metabolism. Furthermore the data indicate that observed effects on the proteome do not strongly correlate with the concentration level used for treatment.
Innovative aspects
• Solid dataset for cardiac cells having been treated with Gliflozins, incl. further aspects as oxidative stress
en
dc.description.sponsorship
European Commission
-
dc.language.iso
en
-
dc.subject
gliflozin
en
dc.subject
cardiomyocytes
en
dc.subject
proteomics
en
dc.title
Investigating the cardioprotective effects of SGLT-2 inhibitors
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
TU Wien, Austria
-
dc.relation.grantno
101034277
-
dc.type.category
Conference Presentation
-
tuw.publication.invited
invited
-
tuw.project.title
Technik für Biowissenschaften Doktoratsstudium
-
tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
-
tuw.researchTopic.id
M6
-
tuw.researchTopic.name
Biological and Bioactive Materials
-
tuw.researchTopic.value
100
-
tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
-
tuw.author.orcid
0000-0002-7071-2316
-
tuw.author.orcid
0000-0003-3950-0312
-
tuw.event.name
APMRS/CEEPC APMRS-CEEPC 2024
-
tuw.event.startdate
23-09-2024
-
tuw.event.enddate
25-09-2024
-
tuw.event.online
On Site
-
tuw.event.type
Event for scientific audience
-
tuw.event.place
Wien
-
tuw.event.country
AT
-
tuw.event.institution
APMA
-
tuw.event.presenter
Hoehlschen, Julia
-
wb.sciencebranch
Chemie
-
wb.sciencebranch
Biologie
-
wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
1060
-
wb.sciencebranch.oefos
3012
-
wb.sciencebranch.value
50
-
wb.sciencebranch.value
20
-
wb.sciencebranch.value
30
-
item.languageiso639-1
en
-
item.openairetype
conference paper not in proceedings
-
item.grantfulltext
none
-
item.fulltext
no Fulltext
-
item.cerifentitytype
Publications
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cp
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
TU Wien
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
