<div class="csl-bib-body">
<div class="csl-entry">Tomin, T., Hofreither, D., Lachmann, J., Nadvornik, M., Jahnel, S., Gollmer, J., Rainer, P. P., Bugger, H., Mendjan, S., Schittmayer-Schantl, M., & Birner-Grünberger, R. (2024, May 2). <i>Pyruvate kinase M2 upon oxidative inury: friend or a foe?</i> [Poster Presentation]. 31st Annual Meeting AAS, St. Gilgen / Wolfgangsee, Austria. http://hdl.handle.net/20.500.12708/207572</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/207572
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dc.description.abstract
Oxidative stress contributes to the functional decline of the myocardium and is correlated with unfavourable metabolic perturbations, including the switch from oxidative metabolism to glycolysis for energy production. Oxidative post-translational modifications (oxPTMs) might play a significant role in this process as they can influence both structure as well as the function of proteins.
We already demonstrated that failing human hearts endure higher levels of oxidative stress and exhibit higher oxidation of several different enzymes, namely related to glucose metabolism. To unravel which of these oxidative changes might be connected with the early onset of oxidative damage, we simulated oxidative injury using cultured cardiomyocytes as well as stem-cell derived and metabolically matured cardioids, followed by redox analysis.
As a result, we report pyruvate kinase M (PKM) as one of the most redox-sensitive enzymes. PKM might be a critical target as a) its fetal isoform, PKM2 was increased in failing hearts, and b) PKM2 was proven susceptible to oxPTM which can lead to inhibition of the enzyme. Treatment of metabolically matured cardioids with TEPP46, a potent PKM2 activator, was unable to hinder the activation of apoptosis upon oxidative injury; however, TEPP46 overall seems to improve cellular antioxidative defence as well as lead to a higher reliance on lipid metabolism (even under hypoxic conditions). Collectively, our data suggest a complex role of PKM in the heart and hints that its enzymatic activity might also be regulated on the post-translational level in cardiac cells.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.description.sponsorship
European Commission
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dc.language.iso
en
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dc.subject
redox stress
en
dc.subject
proteomics
en
dc.subject
failing hearts
en
dc.title
Pyruvate kinase M2 upon oxidative inury: friend or a foe?
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
TU Wien, Austria
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dc.contributor.affiliation
TU Wien, Austria
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dc.contributor.affiliation
Medical University of Graz, Austria
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dc.contributor.affiliation
Medicine - St. Johann in Tirol General Hospital (St. Johann in Tirol, AT)
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dc.contributor.affiliation
Medical University of Graz, Austria
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dc.relation.grantno
F 7309-B21
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dc.relation.grantno
101034277
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dc.type.category
Poster Presentation
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tuw.project.title
Lipidhydrolyse im Krebs und in Lipid-assoziierten Krankheiten
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tuw.project.title
Technik für Biowissenschaften Doktoratsstudium
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tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0000-0002-7071-2316
-
tuw.author.orcid
0000-0003-1147-9179
-
tuw.author.orcid
0000-0002-8734-6359
-
tuw.author.orcid
0000-0002-2840-6454
-
tuw.author.orcid
0000-0002-3524-0405
-
tuw.author.orcid
0000-0001-7539-3951
-
tuw.author.orcid
0000-0003-3249-655X
-
tuw.author.orcid
0000-0003-3950-0312
-
tuw.event.name
31st Annual Meeting AAS
en
tuw.event.startdate
02-05-2024
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tuw.event.enddate
04-05-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
St. Gilgen / Wolfgangsee
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tuw.event.country
AT
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tuw.event.institution
Austrian Atherosclerosis Society
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tuw.event.presenter
Tomin, Tamara
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wb.sciencebranch
Chemie
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch
Anatomie, Pathologie, Physiologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.oefos
3011
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
20
-
wb.sciencebranch.value
30
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item.grantfulltext
none
-
item.fulltext
no Fulltext
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.openairetype
conference poster not in proceedings
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
TU Wien
-
crisitem.author.dept
E163 - Institut für Angewandte Synthesechemie
-
crisitem.author.dept
Medical University of Graz
-
crisitem.author.dept
Medicine - St. Johann in Tirol General Hospital (St. Johann in Tirol, AT)
-
crisitem.author.dept
Medical University of Graz
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0003-1147-9179
-
crisitem.author.orcid
0000-0002-8734-6359
-
crisitem.author.orcid
0000-0002-2840-6454
-
crisitem.author.orcid
0000-0002-3524-0405
-
crisitem.author.orcid
0000-0001-7539-3951
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
