<div class="csl-bib-body">
<div class="csl-entry">Burger, I., Schmal, M., Zimmermann, C., Birner-Grünberger, R., & Schittmayer-Schantl, M. (2024, February 21). <i>Unravelling Fungal RiPPs: Sherlocking with Proteomics, Metabolomics, and a Dash of Molecular Networking Magic</i> [Poster Presentation]. 34th Mass Spectrometry Forum 2024, Wien, Austria.</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/207704
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dc.description.abstract
Among the recognized classes of natural products, ribosomally synthesized and post-translationally modified peptides (RiPPs) represent an appealing group of molecules for advancing therapeutic development due to their biologically active characteristics (e.g., antimicrobial [1], antiviral [2], etc.). In contrast to bacteria [1], there has been limited research so far on RiPPs produced by fungi [3]. Despite the capability of genome mining tools to predict precursor peptide sequences for RiPPs, the extensive and diverse post-translational modifications arising during their maturation make it nearly impossible to anticipate their final structure.
To establish a working protocol, initial experiments were conducted by investigating well-known fungal RiPPs from the Aspergillus flavus fungus. Various strains, including wildtype, knock-out, and overexpression strains of these gene clusters, were examined. Standard proteomics methods adequately identify the precursor peptide and related modifying enzymes within the gene cluster, but they fall short in identifying the fully mature peptide. Instead, comprehensive untargeted metabolomics techniques are employed to identify features displaying altered expression, ideally encompassing the desired mature RiPP. Metabolomics analyses apply both reversed-phase (RP) and hydrophilic interaction chromatography (HILIC), in positive and negative ionization modes, to cover the vast chemical space occupied by RiPPs.
Rather than pinpointing individual metabolites, the approach subjects extracted features to a feature-based molecular network search, resulting in a molecular metabolite network. Given that the amino acid sequence and hence the mass of the RiPP precursor are known, it is envisioned that by analyzing the feature network containing the RiPP precursor, it becomes feasible to track the RiPP intermediates up to the fully mature form. This methodology is planned for adaptation to newly identified potential fungal RiPP candidates.
[1] P. G. Arnison et al., Ribosomally synthesized and post-translationally modified peptide natural products: Overview and recommendations for a universal nomenclature. Nat. Prod. Rep. 2013, 30(1):108-160. doi:10.1039/c2np20085f [2] Y. Fu et al., Antiviral activities and applications of ribosomally synthesized and post-translationally modified peptides (RiPPs). Cellular and Molecular Life Sciences. 2021, 78(8):3921-3940. doi:10.1007/s00018-021-03759-0 [3] S. C. Kessler et al., Out for a RiPP: Challenges and advances in genome mining of ribosomal peptides from fungi. Nat. Prod. Rep. 2022, 39(2):222-230. doi:10.1039/d1np00048a
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
RiPP
en
dc.title
Unravelling Fungal RiPPs: Sherlocking with Proteomics, Metabolomics, and a Dash of Molecular Networking Magic
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
P 34036-B
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dc.type.category
Poster Presentation
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tuw.project.title
Identifizierung und Charakterisierung von RiPPs aus Pilzen
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tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.publication.orgunit
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
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tuw.author.orcid
0000-0001-6419-1270
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tuw.author.orcid
0000-0002-3592-4882
-
tuw.author.orcid
0000-0002-6440-2100
-
tuw.author.orcid
0000-0003-3950-0312
-
tuw.author.orcid
0000-0003-3249-655X
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tuw.event.name
34th Mass Spectrometry Forum 2024
en
tuw.event.startdate
21-02-2024
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tuw.event.enddate
23-02-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
Universität Wien
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tuw.event.presenter
Burger, Isabella
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
30
-
wb.sciencebranch.value
20
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E166-05-1 - Forschungsgruppe Synthetische Biologie und Molekulare Biotechnologie
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.orcid
0000-0001-6419-1270
-
crisitem.author.orcid
0000-0002-3592-4882
-
crisitem.author.orcid
0000-0002-6440-2100
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
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crisitem.author.parentorg
E166-05 - Forschungsbereich Biochemische Technologie
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
