<div class="csl-bib-body">
<div class="csl-entry">Honeder, S., Liesinger, L., Gindlhuber, J., Lindenmann, J., Brcic, L., Schittmayer-Schantl, M., Tomin, T., & Birner-Grünberger, R. (2024, October 8). <i>Deregulated Lipid Hydrolysis in Lung Tumors: Implications for Proliferation and Metabolic Reprogramming in Non-Small Cell Lung Cancer</i> [Poster Presentation]. Cancer Metabolism (EACR), Bilbao, Spain. http://hdl.handle.net/20.500.12708/207724</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/207724
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dc.description.abstract
Lipid metabolism is increasingly recognized as a critical factor in cancer progression, with lipid droplet accumulation emerging as a hallmark of aggressive cancers. However, the role of lipid hydrolysis—specifically the breakdown of stored lipids—in lung cancer remains underexplored. In this study, we aimed to investigate the involvement of lipid hydrolysis enzymes, particularly adipose triglyceride lipase (ATGL) and monoglyceride lipase (MGL), in non-small cell lung cancer (NSCLC).
Our initial investigations involved a comprehensive analysis of tumor and adjacent normal tissue from NSCLC patients using proteomics, lipidomics, and activity-based proteomic profiling (ABPP). We found significant downregulation of several lipid hydrolases, including MGL, in lung tumors compared to normal tissue. Lipidomic profiling revealed substantial triglyceride accumulation and elevated levels of ceramides and lysophosphatidylcholines in tumors, suggesting a disruption in lipid catabolism.
Building on these findings, we explored the functional consequences of lipid hydrolase depletion by generating knockout models of ATGL and MGL in a panel of commonly used NSCLC cell lines. Interestingly, we observed that the deletion of these enzymes led to increased proliferation in some but not all cell lines, which correlated with elevated de-novo fatty acid synthesis. These results suggest that the downregulation of lipid hydrolases may contribute to cancer cell proliferation by altering lipid metabolism.
To further elucidate these mechanisms, ongoing experiments are investigating nutrient utilization, lactate excretion, as well as mitochondrial respiration and glycolysis. Additionally, we are generating cell lines overexpressing lipid hydrolases that were found to be downregulated in tumors to assess reversibility of the observed metabolic changes and its effect on proliferation.
Overall, this study underscores the significant role of lipid hydrolysis in lung cancer metabolism and progression.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
lung cancer
en
dc.subject
proteomics
en
dc.subject
lipidomics
en
dc.title
Deregulated Lipid Hydrolysis in Lung Tumors: Implications for Proliferation and Metabolic Reprogramming in Non-Small Cell Lung Cancer
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Medical University of Graz, Austria
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dc.relation.grantno
F 7309-B21
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dc.type.category
Poster Presentation
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tuw.project.title
Lipidhydrolyse im Krebs und in Lipid-assoziierten Krankheiten
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tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0000-0002-7201-6293
-
tuw.author.orcid
0000-0002-6155-5208
-
tuw.author.orcid
0000-0003-1276-7666
-
tuw.author.orcid
0000-0002-9098-8416
-
tuw.author.orcid
0000-0003-3249-655X
-
tuw.author.orcid
0000-0002-7071-2316
-
tuw.author.orcid
0000-0003-3950-0312
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tuw.event.name
Cancer Metabolism (EACR)
en
tuw.event.startdate
08-10-2024
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tuw.event.enddate
10-10-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Bilbao
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tuw.event.country
ES
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tuw.event.institution
European Association for Cancer Research
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tuw.event.presenter
Honeder, Sophie
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Biologie
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wb.sciencebranch
Anatomie, Pathologie, Physiologie
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wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
3011
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
30
-
wb.sciencebranch.value
20
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item.openairetype
conference poster not in proceedings
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item.fulltext
no Fulltext
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item.grantfulltext
none
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.cerifentitytype
Publications
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crisitem.project.funder
FWF - Österr. Wissenschaftsfonds
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crisitem.project.grantno
F 7309-B21
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
Medical University of Graz
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.orcid
0000-0002-7201-6293
-
crisitem.author.orcid
0000-0002-6155-5208
-
crisitem.author.orcid
0000-0003-1276-7666
-
crisitem.author.orcid
0000-0002-9098-8416
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
