<div class="csl-bib-body">
<div class="csl-entry">Hondl, N., Neubauer, L., Lendl, B., & Ramer, G. (2024, March 13). <i>Advanced Chemical Spectroscopy of Individual Human Milk Extracellular Vesicles</i> [Poster Presentation]. European meeting on InfraRed Nanospectro-Imaging 2024 & EFNS Bruker’s Tech Day, Orsay, France.</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/208065
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dc.description.abstract
Extracellular vesicles (EVs) are nanosized particles enclosed by a lipid bilayer that are released by cells, which are associated with various physiological and pathological functions. EVs are present in all biological fluids and they contain specific cellular components such as lipids, proteins, nucleic acids, RNAs and metabolites. [1, 2] In human milk EVs are believed to be important for the nutrition of infants and for the development of their acquired immunity. State-of-the-art analysis methods are not able to provide label free chemical information at the single vesicle level, hence new analysis techniques are required to study the chemical difference withing EV (sub-)population. [3]
Already existing protocols for AFM-IR do not provide this either. Therefore we introduce a protocol to profile structure and composition of individual EVs beyond the analysis of earlier protocols. [4] First, EVs are selectively immobilized onto a silicone surface using microcontact printed Anti-CD9 antibodies. AFM-IR tapping mode then provides both EV size and mid-infrared spectra of the fragile individual vesicles. In addition, AFM-IR images provide spectral information for multiple EVs within a single measurement. Stacking images taken at different wavenumbers yields hyperspectral chemical images of vesicles at nanoscale spatial resolution. Chemometrics is used to break down the chemical composition of the individual EVs.
In this work we demonstrated chemical analysis of individual extracellular vesicles with our new established protocol. Nanoscale AFM-IR spectra of EVs compare favourably to bulk reference spectra. Principal component analysis (PCA) was applied to find heterogeneity of composition within individual. Due to the EV size information provided by AFM, it is possible to study correlations between vesicle size and their chemical composition.
References
[1] Hessvik, N.P., et al. ”Current knowledge on exosome biogenesis and release.” Cell. Mol. Life Sci. 75, 193–208 (2018).
[2] Daniella Schatz, et al. “Extracellular vesicles — new players in cell–cell communication in aquatic environments” Current Opinion in Microbiology, Volume 43 148-154 (2018)
[3] Théry, Clotilde, et al. "Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines." Journal of extracellular vesicles 7.1 (2018)
[4] Kim, S. Y., et al. “None of us is the same as all of us: resolving the heterogeneity of extracellular vesicles using single-vesicle, nanoscale characterization with resonance enhanced atomic force microscope infrared spectroscopy (AFM-IR). “, Nanoscale Horizons, (2018)
en
dc.description.sponsorship
European Commission
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dc.language.iso
en
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dc.subject
Extracellular Vesicles
en
dc.subject
AFM-IR
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dc.subject
Chemometrics
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dc.title
Advanced Chemical Spectroscopy of Individual Human Milk Extracellular Vesicles
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
953234
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dc.type.category
Poster Presentation
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tuw.project.title
Tumor und Lymphknoten auf einer Chip Plattform für Krebsstudien
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tuw.researchinfrastructure
Pilotfabrik
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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tuw.author.orcid
0009-0001-9282-4474
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tuw.author.orcid
0000-0003-3838-5842
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tuw.author.orcid
0000-0001-8307-5435
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tuw.event.name
European meeting on InfraRed Nanospectro-Imaging 2024 & EFNS Bruker's Tech Day
en
tuw.event.startdate
12-03-2024
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tuw.event.enddate
15-03-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Orsay
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tuw.event.country
FR
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tuw.event.institution
Universite Paris-Saclay
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tuw.event.presenter
Hondl, Nikolaus
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
restricted
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
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crisitem.author.orcid
0009-0001-9282-4474
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crisitem.author.orcid
0009-0002-9359-1841
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crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
