<div class="csl-bib-body">
<div class="csl-entry">Hofreither, D., Hofer, L., Liesinger, L., Bednárová Schäpertöns, V., Stor, J., Borth, N., & Birner-Grünberger, R. (2024, June 23). <i>Time-Resolved Mass Spectrometry-Based Multi-Omics Characterisation of NISTCHO Cellular Function In Response To Nutrient Influx</i> [Poster Presentation]. ESACT 2024, Edinburgh, United Kingdom of Great Britain and Northern Ireland (the). http://hdl.handle.net/20.500.12708/208068</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/208068
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dc.description.abstract
Relevance to Theme Selected
“Big data” approaches, such as multi-omics-based characterisation of cellular function, show great potential in aiding academic and industrial endeavours to improve therapeutics production.
Impact/Novelty
The utilisation of state-of-the-art host cell-omics to supplement conventional, medium-based measurements as proxies for cellular function will further the understanding of producer CHO cell lines.
Introduction
To meet the growing demand for recombinant biotherapeutics employed today, producer cell lines are under ever-constant development to improve their productivity, stability and product quality. Accurate analysis of protein expression and post-translational modification of proteins by reversible phosphorylation can effectively decipher vital cellular processes and their regulation.
Methods/Approach
The development of a novel, fast, and representative multi-omics sampling approach for suspension cultures allowing analysis of the host-cell proteome, phosphoproteome, metabolome, and secretome was carried out. To demonstrate the application of this approach, a fed-batch culture of the openly available producer cell line NISTCHO was consequently investigated for the impact of feeding on cellular function. Sampling took place in both the exponential and stationary growth phases to reflect the most critical stages of the bioprocess. Results were integrated into the parallel monitoring of cell proliferation and viability, as well as nutrient consumption and metabolic excrements.
Results
Analysis of bioprocess parameters and host cell-omic datasets of multiple time points before and after scheduled feeding of NISTCHO revealed distinct responses to altered nutrient availability. This work provides time-resolved and highly comprehensive profiling of the acute impact of feeding on the cellular landscape, including the proteome and phosphoproteome.
Conclusion
A novel mass spectrometry-based multi-omics method can offer an in-depth analysis of cellular function in various product and/or process-relevant biological settings. The advanced characterisation of an academically available producer cell line will drive research in recombinant protein production and bioprocess engineering.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
CHO host cell
en
dc.subject
multi-omics
en
dc.subject
monoclonal antibody production
en
dc.title
Time-Resolved Mass Spectrometry-Based Multi-Omics Characterisation of NISTCHO Cellular Function In Response To Nutrient Influx
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
BOKU University, Austria
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dc.contributor.affiliation
BOKU University, Austria
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dc.contributor.affiliation
BOKU University, Austria
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dc.relation.grantno
FG 1200-N
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dc.type.category
Poster Presentation
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tuw.project.title
Digitalized Production of Therapeutics
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0009-0006-2194-706X
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tuw.author.orcid
0000-0001-8076-3187
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tuw.author.orcid
0000-0001-6324-9338
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tuw.author.orcid
0000-0003-3950-0312
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tuw.event.name
ESACT 2024
en
tuw.event.startdate
23-06-2024
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tuw.event.enddate
26-06-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Edinburgh
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tuw.event.country
GB
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tuw.event.presenter
Hofreither, Dominik
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wb.sciencebranch
Chemie
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wb.sciencebranch
Industrielle Biotechnologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
2090
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wb.sciencebranch.value
50
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wb.sciencebranch.value
50
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item.openairetype
conference poster not in proceedings
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item.cerifentitytype
Publications
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item.grantfulltext
none
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.fulltext
no Fulltext
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crisitem.project.funder
FWF - Österr. Wissenschaftsfonds
-
crisitem.project.grantno
FG 1200-N
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
BOKU University
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
BOKU University
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crisitem.author.dept
BOKU University
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.orcid
0009-0006-2194-706X
-
crisitem.author.orcid
0000-0001-8076-3187
-
crisitem.author.orcid
0000-0001-6324-9338
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
