<div class="csl-bib-body">
<div class="csl-entry">Hofreither, D., Tomin, T., Mendjan, S., Schittmayer-Schantl, M., & Birner-Grünberger, R. (2024, June 11). <i>Elucidation of the Effects of Metabolic Agents on the Cardiac Proteome and Redox Landscape</i> [Poster Presentation]. 38th Meeting of the European Section of the ISHR, Toulouse, France.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/208210
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dc.description.abstract
The interface between metabolic disorders and heart disease is an active area of research, with diabetes, metabolic syndrome, ageing, and obesity recognised as significant risk factors for impaired cardiac function. Metabolic agents such as metformin, myo-inositol and berberine have traditionally been prescribed or otherwise utilised for improving insulin sensitivity and glucose homeostasis. Epidemiological data and early clinical trials suggest cardiovascular-related benefits of these compounds in the respective populations, but little is known about their effects on cardiac cells. We therefore set out to characterise the direct impact on cardiomyocytes beyond obvious benefits to systemic health. As aberrant metabolism and oxidative stress play a major role in the development and progression of these interrelated pathologies, a specific focus was placed on changes in the redox landscape. Using state-of-the-art mass spectrometry and preservative two-step cysteine alkylation, proteomic and redox-proteomic characterisation of these compounds was performed in AC16 human cardiomyocytes. Complementary glutathione status was assessed as a cellular marker of oxidative stress. Initial observations, particular to antioxidative capacity, were further explored in an ischaemia-reperfusion injury model employing hiPSC-derived cardiac organoids, providing additional clinical and in vivo context. Here we provide a comprehensive depiction of the cardiac proteome and redox state following treatment with commonly applied metabolic agents at pharmacologically and clinically relevant concentrations. Notably, we show the impact of myo-inositol on redox-sensitive active sites of PKM and FASN, key regulators of cellular metabolism that were previously identified in the tissue of failing hearts. Additional validation in a model of reperfusion injury highlights the potential cardioprotective effects by preserving metabolic capacity and mediating the stress response to ensure continued cellular function. These findings provide a solid foundation for future investigations into clinical applications.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
myo-inositol
en
dc.subject
heart
en
dc.subject
redox-proteomics
en
dc.title
Elucidation of the Effects of Metabolic Agents on the Cardiac Proteome and Redox Landscape
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
F 7309-B21
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dc.type.category
Poster Presentation
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tuw.project.title
Lipidhydrolyse im Krebs und in Lipid-assoziierten Krankheiten
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tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0000-0002-7071-2316
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tuw.author.orcid
0000-0001-7539-3951
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tuw.author.orcid
0000-0003-3249-655X
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tuw.author.orcid
0000-0003-3950-0312
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tuw.event.name
38th Meeting of the European Section of the ISHR
en
tuw.event.startdate
11-06-2024
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tuw.event.enddate
14-06-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Toulouse
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tuw.event.country
FR
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tuw.event.presenter
Hofreither, Dominik
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wb.sciencebranch
Chemie
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch
Anatomie, Pathologie, Physiologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.oefos
3011
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wb.sciencebranch.value
50
-
wb.sciencebranch.value
30
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wb.sciencebranch.value
20
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item.languageiso639-1
en
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item.openairetype
conference poster not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0001-7539-3951
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
