<div class="csl-bib-body">
<div class="csl-entry">Riedlsperger, L., Dabrowska, A., Gazizov, I., & Lendl, B. (2024, March 12). <i>Optimizing Protein Analysis with QCL-based Multi-Pathlength Mid-IR Spectroscopy</i> [Conference Presentation]. AK-PAT 17. Interdisziplinäres Doktorandenseminar, Freiburg im Breisgau, Germany. http://hdl.handle.net/20.500.12708/208956</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/208956
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dc.description.abstract
Mid-infrared spectroscopy, with its fixed transmission pathlength design, has long been a well-established method for the analysis of proteins and polypeptides in solution. Ideally, a solvent’s transmission should be around 1/e, correlating to an optimal absorbance of ~0.4 (or log10e). However, when dealing with strong absorbing matrix like water, the varied background absorbance across different wavenumber regions proves to be a challenge. Proteins, characterized by their unique amide I (1700 – 1600 cm-1) and amide II (1600 – 1500 cm-1) bands, are especially difficult to distinguish with spectroscopic analysis in aqueous solutions due to the overlapping bending vibration of water. Our research introduces a system that measures samples at multiple pathlengths simultaneously to determine the most optimal pathlength for each characteristic band of the solute. To achieve this, we employ an external-cavity quantum cascade laser (EC-QCL) that serves as a powerful source of IR radiation. The laser beam is subsequently expanded to a line and guided through the wedge-shaped transmission cell with pathlengths ranging from ~10 µm to 400 µm. A pyroelectric linear array, consisting of 256 elements, detects then the transmitted radiation. We aim to employ caffeine in water as a model sample system to illustrate the variability in optimal pathlengths depending on the absorbance of the solvent. The hereby proposed configuration combines the advantages of QCLs over conventional thermal emitters while also providing the capability to cover a wider dynamic range of absorbance values. Consequently, it is possible to increase the signal-to-noise ratio for measurements of proteins in aqueous solutions and provide greater insight into the investigated sample.
en
dc.description.sponsorship
European Commission
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dc.language.iso
en
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dc.subject
Protein analysis
en
dc.subject
IR spectroscopy of aqueous solutions
en
dc.subject
quantum cascade laser
en
dc.subject
Multi-pathlength transmission spectroscopy
en
dc.title
Optimizing Protein Analysis with QCL-based Multi-Pathlength Mid-IR Spectroscopy
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
101119714
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dc.type.category
Conference Presentation
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tuw.project.title
Advanced, trustworthy AI and data solutions for individualised automated milking & feeding of dairy cows
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tuw.researchTopic.id
E4
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Environmental Monitoring and Climate Adaptation
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tuw.researchTopic.name
Beyond TUW-research focus
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tuw.researchTopic.value
10
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tuw.researchTopic.value
90
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tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
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tuw.author.orcid
0009-0009-7577-4397
-
tuw.author.orcid
0000-0002-3074-5674
-
tuw.author.orcid
0000-0002-7143-4039
-
tuw.author.orcid
0000-0003-3838-5842
-
tuw.event.name
AK-PAT 17. Interdisziplinäres Doktorandenseminar
de
tuw.event.startdate
11-03-2024
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tuw.event.enddate
13-11-2024
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Freiburg im Breisgau
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tuw.event.country
DE
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tuw.event.institution
Fraunhofer-Institut für Physikalische Messtechnik
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tuw.event.presenter
Riedlsperger, Lisa
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.languageiso639-1
en
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item.openairetype
conference paper not in proceedings
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item.grantfulltext
none
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item.fulltext
no Fulltext
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item.cerifentitytype
Publications
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.orcid
0009-0009-7577-4397
-
crisitem.author.orcid
0000-0002-3074-5674
-
crisitem.author.orcid
0000-0002-7143-4039
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
