Spore inoculum quality in filamentous bioprocesses is a critical parameter associated with viable spore concentration (1) and spore germination (2). It influences pellet morphology and, consequently, process performance. The state-of-the-art method to measure viable spore concentration is tedious, associated with significant inherent bias, and not applicable in real-time. Therefore, it is not usable as process analytical technology (PAT). Spore germination has so far been monitored using image analysis, which is hampered by complex medium background often observed in filamentous bioprocesses. The method presented here is based on the combination of viability staining and large-particle flow cytometry which enables measurements in real-time and hence aims to be applicable as a PAT tool. It is compatible with the complex media background and allows the quantification of metabolically active spores and the monitoring of spore germination. A distinction of germinated spores and not germinated spores was based on logistic regression, using multiparameteric data from flow cytometry. In a first step, a significant correlation between colony-forming unit (CFU) counts and viable spore concentration (1) in an industrially relevant model bioprocess was found. Spore germination (2) was followed over the initial process phase with close temporal resolution. The validation of the method showed an error below 5 %. Differences in spore germination for various spore inocula ages and spore inoculum concentrations were monitored. The real-time applicability of the method suggests the implementation as a PAT tool in filamentous bioprocesses.
en
dc.language
English
-
dc.language.iso
en
-
dc.publisher
Springer
-
dc.relation.ispartof
Applied Microbiology and Biotechnology
-
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
-
dc.subject
Filamentous fungi
en
dc.subject
Flow cytometry
en
dc.subject
Spore germination
en
dc.subject
Spore viability
en
dc.subject
Process analytical technology
en
dc.title
At-line determining spore germination of Penicillium chrysogenum bioprocesses in complex media
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.description.startpage
8923
-
dc.description.endpage
8930
-
dc.rights.holder
# The Author(s) 2016
-
dc.type.category
Original Research Article
-
tuw.container.volume
100
-
tuw.container.issue
20
-
tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.version
vor
-
dcterms.isPartOf.title
Applied Microbiology and Biotechnology
-
tuw.publication.orgunit
E166 - Inst. f. Verfahrenstechnik, Umwelttechnik und Techn. Biowissenschaften
-
tuw.publisher.doi
10.1007/s00253-016-7787-y
-
dc.date.onlinefirst
2016-08-24
-
dc.identifier.eissn
1432-0614
-
dc.identifier.libraryid
AC11360823
-
dc.description.numberOfPages
8
-
dc.identifier.urn
urn:nbn:at:at-ubtuw:3-2594
-
tuw.author.orcid
0000-0003-2314-1458
-
dc.rights.identifier
CC BY 4.0
de
dc.rights.identifier
CC BY 4.0
en
wb.sci
true
-
item.languageiso639-1
en
-
item.cerifentitytype
Publications
-
item.cerifentitytype
Publications
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cf
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cf
-
item.fulltext
with Fulltext
-
item.openaccessfulltext
Open Access
-
item.grantfulltext
open
-
item.openairetype
Article
-
item.openairetype
Artikel
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften