<div class="csl-bib-body">
<div class="csl-entry">Engel, N. Y., Weiss, V., Marchetti-Deschmann, M., & Allmaier, G. (2017). nES GEMMA analysis of lectins and their interactions with glycoproteins – separation, detection, and sampling of noncovalent biospecific complexes. <i>Journal of The American Society for Mass Spectrometry</i>. https://doi.org/10.1007/s13361-016-1483-0</div>
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In order to better understand biological events, lectin–glycoprotein interactions are of interest. The possibility to gather more information than the mere positive or negative response for interactions brought mass spectrometry into the center of many research fields. The presented work shows the potential of a nano-electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA) to detect weak, noncovalent, biospecific interactions besides still unbound glycoproteins and unreacted lectins without prior liquid phase separation. First results for Sambucus nigra agglutinin, concanavalin A, and wheat germ agglutinin and their retained noncovalent interactions with glycoproteins in the gas phase are presented. Electrophoretic mobility diameters (EMDs) were obtained by nES GEMMA for all interaction partners correlating very well with molecular masses determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of the individual molecules. Moreover, EMDs measured for the lectin–glycoprotein complexes were in good accordance with theoretically calculated mass values. Special focus was laid on complex formation for different lectin concentrations and binding specificities to evaluate the method with respect to results obtained in the liquid phase. The latter was addressed by capillary electrophoresis on-a-chip (CE-on-a-chip). Of exceptional interest was the fact that the formed complexes could be sampled according to their size onto nitrocellulose membranes after gas-phase separation. Subsequent immunological investigation further proved that the collected complex actually retained its native structure throughout nES GEMMA analysis and sampling.
en
dc.description.sponsorship
Austrian Science Foundation (FWF)
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dc.language
English
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dc.language.iso
en
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dc.publisher
ACS Publications
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dc.relation.ispartof
Journal of The American Society for Mass Spectrometry
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dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
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dc.subject
Lectin
en
dc.subject
Glycoproteinn
en
dc.subject
ES GEMMA
en
dc.subject
CE-on-a-chip
en
dc.subject
Electrophoresis
en
dc.title
nES GEMMA analysis of lectins and their interactions with glycoproteins – separation, detection, and sampling of noncovalent biospecific complexes
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.relation.grantno
TRP 29-N20
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dcterms.dateSubmitted
2016-04-29
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dc.rights.holder
The Author(s), 2016
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dc.type.category
Original Research Article
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tuw.journal.peerreviewed
true
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tuw.peerreviewed
true
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tuw.version
vor
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dcterms.isPartOf.title
Journal of The American Society for Mass Spectrometry
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tuw.publication.orgunit
E164 - Institut für Chemische Technologien und Analytik
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tuw.publisher.doi
10.1007/s13361-016-1483-0
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dc.date.onlinefirst
2016-09-19
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dc.identifier.eissn
1879-1123
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dc.identifier.libraryid
AC11360826
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dc.description.numberOfPages
10
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dc.identifier.urn
urn:nbn:at:at-ubtuw:3-2604
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tuw.author.orcid
0000-0002-0056-6819
-
tuw.author.orcid
0000-0002-8060-7851
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tuw.author.orcid
0000-0002-1438-9462
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dc.rights.identifier
CC BY 4.0
de
dc.rights.identifier
CC BY 4.0
en
wb.sci
true
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item.openaccessfulltext
Open Access
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item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
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open
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item.languageiso639-1
en
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item.openairetype
research article
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item.fulltext
with Fulltext
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item.cerifentitytype
Publications
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crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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crisitem.author.orcid
0000-0002-0056-6819
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crisitem.author.orcid
0000-0002-8060-7851
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crisitem.author.orcid
0000-0002-1438-9462
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crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
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crisitem.author.parentorg
E150 - Fakultät für Technische Chemie
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crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie