<div class="csl-bib-body">
<div class="csl-entry">Weiss, V., Weber, V., & Marchetti-Deschmann, M. (2025, April 25). <i>Characterizing EV preparations via gas-phase electrophoresis on a nES GEMMA instrumentation</i> [Poster Presentation]. ISEV 2025 Annual Meeting Vienna, Vienna, Austria. http://hdl.handle.net/20.500.12708/215824</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/215824
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dc.description
https://www.isev.org/isev-annual-meeting
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dc.description.abstract
Characterizing EV preparations via gas-phase electrophoresis
on a nES GEMMA instrumentation
Victor U. Weiss1, Viktoria Weber2, Martina Marchetti-Deschmann1
1 TU Wien, Institute of Chemical Technologies and Analytics, Vienna, Austria
2 University of Continuing Education Krems, Krems, Austria
Introduction: As recommended by the European Commission, nanoparticle characterization should be based on particle number concentrations (2011/696/EU from October 18th, 2011, updated in 2022). Gas-phase electrophoresis on a nano electrospray gas-phase electrophoretic mobility molecular analyser (nES GEMMA) allows such an approach. Single-charged (bio-)nanoparticles obtained after a nES process with subsequent surface-drying and charge equilibration are separated based on their electrophoretic mobility diameter (EMD) corresponding to the particle diameter for spherical analytes. By voltage variation, particles of different EMD successively reach the instruments’ detector unit and are counted, resulting in a spectrum in the size range of several few up to several hundred nm EMD.
Methods: EVs were either purified via ultracentrifugation or a combination of precipitation and centrifugation. Subsequently, sample buffer was exchanged to volatile ammonium acetate. EV monitoring was via nanoparticle tracking analysis (NTA, Particle Metrix, Inning am Ammersee, Germany), gas-phase electrophoresis was on a nES GEMMA instrumentation (TSI Inc., Shoreview, MN, USA).
Results: In previous work, we succeeded in demonstrating the applicability of nES GEMMA for the analysis of liposomes, vesicles consisting of a lipid bilayer encapsulating an aqueous lumen. nES GEMMA enabled the characterization of vesicles in terms of size distribution, particle number concentration and the occurrence of smaller-sized building blocks next to large vesicles. Also, offline hyphenation of gas-phase electrophoresis with orthogonal analysis methods, for instance atomic force microscopy, MALDI mass spectrometry or spectroscopy was shown.
In vivo, cell-derived EVs, being part of cell/cell communication, are comparable to liposomes. They are envisioned as pharmaceutical cargo transporters, explaining the need for their characterization. We succeeded in porting gas-phase electrophoresis of liposomes to the characterization of EVs, demonstrating protein co-purification and loss of EV stability upon further polishing of vesicle preparations. Our nES GEMMA results were compared to data from NTA.
Summary: We demonstrate gas-phase electrophoresis relating additional characteristics for EV containing samples in comparison to prevailing techniques like e.g. NTA.
en
dc.language.iso
en
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dc.subject
nES GEMMA
en
dc.subject
gas-phase electrophoresis
en
dc.subject
extracellular vesicles
en
dc.subject
EVs
en
dc.subject
liposomes
en
dc.title
Characterizing EV preparations via gas-phase electrophoresis on a nES GEMMA instrumentation
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Universität für Weiterbildung Krems, Austria
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dc.type.category
Poster Presentation
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tuw.researchTopic.id
M2
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Materials Characterization
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
40
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tuw.researchTopic.value
60
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tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
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tuw.publication.orgunit
E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials
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tuw.publication.orgunit
E056-12 - Fachbereich ENROL DP
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tuw.publication.orgunit
E056-14 - Fachbereich Mature Tissue
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tuw.author.orcid
0000-0002-0056-6819
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tuw.author.orcid
0000-0002-3963-9077
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tuw.author.orcid
0000-0002-8060-7851
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tuw.event.name
ISEV 2025 Annual Meeting Vienna
en
tuw.event.startdate
24-04-2025
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tuw.event.enddate
27-04-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Vienna
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tuw.event.country
AT
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tuw.event.institution
International Society of Extracellular Vesicles (ISEV)
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tuw.event.presenter
Weiss, Victor
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tuw.event.track
Multi Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.openairetype
conference poster not in proceedings
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item.cerifentitytype
Publications
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item.grantfulltext
restricted
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.fulltext
no Fulltext
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crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
Universität für Weiterbildung Krems
-
crisitem.author.dept
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.orcid
0000-0002-0056-6819
-
crisitem.author.orcid
0000-0002-8060-7851
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
