<div class="csl-bib-body">
<div class="csl-entry">Kopittke, C., Gaugutz, A., Paul Matthias, Lagler Richard, Schrangl, L., Schütz, G., & Brameshuber, M. (2025, July 1). <i>Immuno-modulating Effect of Temperature on Early T cell Activation</i> [Poster Presentation]. 15th European Biophysics Congress (EBSA 2025), Rom, Italy. http://hdl.handle.net/20.500.12708/216910</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/216910
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dc.description.abstract
Changes in body temperature influence our immune system. While increased temperatures (e.g. during fever) can have stimulating effects, anti-inflammatory treatments induce low temperatures to supress the immune response. Although this is well-established in medical treatments, details on the cellular or molecular mechanisms of thermic immuno-modulation remain elusive.
To investigate how temperature affects early T cell signalling, we studied primary murine CD4+ T cells. For precise temperature control, we functionalized supported lipid bilayers (SLBs) on microscopy-compatible, heatable glass substrates (VAHEAT, Interherence). Mimicking the surface of antigen presenting cells, the SLBs provide cognate antigen, costimulatory and adhesion proteins which induce specific T cell activation. Via ratiometric calcium imaging, we compare T cell behaviour in hypo-, normo- and hyperthermic conditions, revealing temperature effects on cell migration, antigen recognition and Ca2+ levels within cells. Additionally, we show how elevated temperatures damage protein-functionalized SLBs and present strategies to protect SLB integrity.
en
dc.language.iso
en
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dc.subject
T-cell activation
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dc.subject
Temperature
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dc.subject
Supported Lipid Bilayers
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dc.title
Immuno-modulating Effect of Temperature on Early T cell Activation
