<div class="csl-bib-body">
<div class="csl-entry">Gaugutz, A., Velas, L., Georgiou, E., Xing, Y., Moser, S., Maloberti Julian, Jesacher, A., Howorka, S., & Schütz, G. (2025, July 3). <i>3D Single Molecule Tracking of Membrane-Bound Proteins and DNA Nanoprobes using Defocused Imaging</i> [Poster Presentation]. 15th European Biophysics Congress (EBSA 2025), Rom, Italy. http://hdl.handle.net/20.500.12708/217197</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/217197
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dc.description.abstract
Protein movement and biomolecular interactions at cellular interfaces are strongly influenced by the nanoscale topography of biological membranes. Tracking individual membrane proteins within this complex cellular 3D environment at high spatial resolution is essential to understand biological mechanisms. While standard single molecule localization microscopy (SMLM) provides high localisation precision in lateral dimensions, its axial performance is limited. To achieve high localisation precision also in axial dimension, we present a 3D SMLM approach which uses defocused imaging to track single molecules in all three dimensions with an axial resolution down to 11 nm, as demonstrated with two fluorophore-tagged DNA nanoprobes on supported lipid bilayers. We also follow the 3D motion of T cell receptors in live cells, capturing their diffusion characteristics in activating and resting conditions. Our results lay the foundation for high-resolution 3D tracking of molecules, enabling new insights into the spatial organisation and dynamics of cellular processes.
en
dc.language.iso
en
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dc.subject
DNA Origami
en
dc.subject
Single Molecules
en
dc.subject
Fluorescence Microscopy
en
dc.title
3D Single Molecule Tracking of Membrane-Bound Proteins and DNA Nanoprobes using Defocused Imaging
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University College London, United Kingdom of Great Britain and Northern Ireland (the)
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dc.contributor.affiliation
University College London, United Kingdom of Great Britain and Northern Ireland (the)
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dc.contributor.affiliation
Innsbruck Medical University, Austria
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dc.contributor.affiliation
Innsbruck Medical University, Austria
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dc.contributor.affiliation
University College London, United Kingdom of Great Britain and Northern Ireland (the)
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dc.type.category
Poster Presentation
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E134-04 - Forschungsbereich Biophysics
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tuw.author.orcid
0000-0003-1542-1089
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tuw.event.name
15th European Biophysics Congress (EBSA 2025)
en
tuw.event.startdate
30-06-2025
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tuw.event.enddate
04-07-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Rom
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tuw.event.country
IT
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tuw.event.presenter
Gaugutz, Anna
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wb.sciencebranch
Chemie
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wb.sciencebranch
Physik, Astronomie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
1030
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wb.sciencebranch.value
50
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wb.sciencebranch.value
50
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item.grantfulltext
restricted
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.openairetype
conference poster not in proceedings
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item.languageiso639-1
en
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crisitem.author.dept
E134-04 - Forschungsbereich Biophysics
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crisitem.author.dept
E134-04 - Forschungsbereich Biophysics
-
crisitem.author.dept
University College London, United Kingdom of Great Britain and Northern Ireland (the)
-
crisitem.author.dept
University College London, United Kingdom of Great Britain and Northern Ireland (the)
-
crisitem.author.dept
Innsbruck Medical University, Austria
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crisitem.author.dept
Innsbruck Medical University, Austria
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crisitem.author.dept
University College London, United Kingdom of Great Britain and Northern Ireland (the)
