<div class="csl-bib-body">
<div class="csl-entry">Weiss, V., Miskovic, M. Z., Petr Kuban, Lado, S., Steininger, C., & Marchetti-Deschmann, M. (2025, September 4). <i>Gas-phase electrophoresis (nES GEMMA instrumentation) of exhaled breath condensate (EBC)</i> [Conference Presentation]. Euroanalysis 2025, Barcelona, Spain.</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/219097
-
dc.description.abstract
Human breath analysis is an attractive alternative strategy for non-invasive retrieval of representative, unbiased samples directly from the lung of a patient. In such a way, information on the health status of a person can be obtained. Significant effort has been put e.g. into volatile organic compounds (VOCs) that show characteristic patterns in lung cancer-related breath samples. Also, VOC analysis
via GC-MS has been lately used for SARS-CoV-2 diagnostics.
Gas-phase electrophoresis on a nano electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA) also known as nES differential mobility analyzer (nES DMA) enables the separation and detection of (bio-)material in the size range from several few to several hundred nm
diameter. [1] Analytes are electrosprayed from a volatile, liquid electrolyte solution, followed by drying of droplets and concomitant charge equilibration in a bipolar atmosphere sinduced e.g. by a 210Poalpha-particle source, a corona discharge process, or similar. Subsequently, singly-charged analytes are separated according to their surface-dry particle size (electrophoretic mobility, EM, diameter) in a
high laminar sheath flow of particle free air and a tunable electric field applied in an orthogonal direction. Particles are counted after separation yielding true particle-number concentration values in accordance with a recommendation of the EC for nanoparticle characterization. Finally, a spectrum of a corresponding sample relating particle size and respective number values is obtained. Based on
our previous experience with the analysis of proteins, extracellular vesicles, viruses and virus-like particles we reasoned that such an instrument is also capable to contribute to the characterization of particles in exhaled air (PEx), in particular in exhaled breath condensate (EBC).
Indeed, by applying EBC samples to our nES GEMMA instrument we were successful in obtaining corresponding spectra. For one donor, recorded spectra remained more or less constant over time (different time points of EBC collection). Low sample conductivity made as stable as possible nES conditions in terms of recorded current values a necessary prerequisite for data interpretation.
Expanding our sampling strategy to more donors yielded spectra, some of which differed between individual donors and could be clustered via hierarchical cluster analysis. The overall biological and clinical relevance of recorded spectra will have to be targeted in a follow-up study. Likewise, this follow-up study will have to focus on clearly standardized collection, sample storage and
measurement conditions.
Experiments presented in this study indicate that PEx detected during our experiments corresponds to unspecific aggregates. Particle collection together with further MALDI MS analysis will help in the future to deepen our knowledge on PEx.
en
dc.language.iso
en
-
dc.subject
exhaled breath
en
dc.subject
EBC
en
dc.subject
gas-phase electrophoresis
en
dc.subject
nES GEMMA
en
dc.subject
nES DMA
en
dc.title
Gas-phase electrophoresis (nES GEMMA instrumentation) of exhaled breath condensate (EBC)
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Zagreb, Croatia
-
dc.contributor.affiliation
Medical University of Vienna, Austria
-
dc.type.category
Conference Presentation
-
tuw.researchTopic.id
M2
-
tuw.researchTopic.id
M6
-
tuw.researchTopic.name
Materials Characterization
-
tuw.researchTopic.name
Biological and Bioactive Materials
-
tuw.researchTopic.value
25
-
tuw.researchTopic.value
75
-
tuw.publication.orgunit
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
tuw.publication.orgunit
E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials
-
tuw.publication.orgunit
E056-12 - Fachbereich ENROL DP
-
tuw.publication.orgunit
E056-14 - Fachbereich Mature Tissue
-
tuw.author.orcid
0000-0002-0056-6819
-
tuw.author.orcid
0000-0002-3959-6396
-
tuw.author.orcid
0000-0003-3500-7205
-
tuw.author.orcid
0000-0002-8060-7851
-
tuw.event.name
Euroanalysis 2025
en
tuw.event.startdate
31-08-2025
-
tuw.event.enddate
04-09-2025
-
tuw.event.online
On Site
-
tuw.event.type
Event for scientific audience
-
tuw.event.place
Barcelona
-
tuw.event.country
ES
-
tuw.event.presenter
Weiss, Victor
-
tuw.event.track
Multi Track
-
wb.sciencebranch
Chemie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.value
100
-
item.languageiso639-1
en
-
item.openairetype
conference paper not in proceedings
-
item.openairecristype
http://purl.org/coar/resource_type/c_18cp
-
item.grantfulltext
restricted
-
item.cerifentitytype
Publications
-
item.fulltext
no Fulltext
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.dept
University of Zagreb, Croatia
-
crisitem.author.dept
Medical University of Vienna, Austria
-
crisitem.author.dept
E164-01-1 - Forschungsgruppe Massenspektrometrische Bio- und Polymeranalytik
-
crisitem.author.orcid
0000-0002-0056-6819
-
crisitem.author.orcid
0000-0002-3959-6396
-
crisitem.author.orcid
0000-0003-3500-7205
-
crisitem.author.orcid
0000-0002-8060-7851
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
