<div class="csl-bib-body">
<div class="csl-entry">Hofreither, D., Hofer, L., Rauter, T., Liesinger, L., Berger, T., Schäpertöns, Fortelny, N., Huber, C., Schittmayer-Schantl, M., Borth, N., & Birner-Grünberger, R. (2025, September 12). <i>Temporal Profiling of Cellular Responses to Nutrient Feeding Using Integrated Multi-Omics in the NIST-CHO Reference Bioprocess</i> [Conference Presentation]. APMRS 2025, Wien, Austria. http://hdl.handle.net/20.500.12708/219595</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/219595
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dc.description.abstract
Producer cell lines are continuously optimised to meet rising biotherapeutic demand. Insights gained from host-cell omics, complementing conventional bioprocess monitoring, may enable predictable product quality and the identification of critical process parameters.
A bioprocess-integrated multi-omics approach addressing multiple key challenges was designed and evaluated, enabling parallel investigation of the host-cell proteome, phosphoproteome, metabolome, and secretome. To demonstrate applicability, a fed-batch culture of the open-access NISTCHO reference cell line was examined for protein and phosphorylation dynamics post-feeding. Simultaneous monitoring of nutrient consumption, metabolic excretions, product titer, and antibody quality attributes was conducted. Additionally, an in-house R package was developed to streamline large-scale time-course data analysis, enabling comprehensive identification of dynamic features and clustering of temporal behaviour.
This study profiles over 7000 proteins and 10000 phosphorylation sites in response to altered nutrient availability around feeding windows distinct to different phases of the bioprocess. Identified signatures span regulators of redox homeostasis and metabolism, include key glycosylation-related enzymes directly impacting product attributes, and pinpoint regulatory phosphorylation sites governing stress responses, demonstrating the capability of this approach for high-quality bioprocess reference data generation.
en
dc.language.iso
en
-
dc.subject
CHO cell culture
en
dc.subject
proteomics
en
dc.subject
phosphoproteomics
en
dc.subject
monoclonal antibody
en
dc.subject
bioprocess
en
dc.title
Temporal Profiling of Cellular Responses to Nutrient Feeding Using Integrated Multi-Omics in the NIST-CHO Reference Bioprocess
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
Department of Biotechnology - BOKU University (Vienna, AT)
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dc.contributor.affiliation
University of Salzburg (Salzburg, AT)
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dc.contributor.affiliation
University of Salzburg, Austria
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dc.contributor.affiliation
University of Salzburg, Austria
-
dc.contributor.affiliation
University of Salzburg, Austria
-
dc.contributor.affiliation
University of Salzburg, Austria
-
dc.contributor.affiliation
Department of Biotechnology - Universität für Bodenkultur Wien (Wien, AT)
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.author.orcid
0009-0006-2194-706X
-
tuw.author.orcid
0009-0004-5578-3628
-
tuw.author.orcid
0000-0001-8076-3187
-
tuw.author.orcid
0000-0003-4025-9968
-
tuw.author.orcid
0000-0003-3249-655X
-
tuw.author.orcid
0000-0001-6324-9338
-
tuw.author.orcid
0000-0003-3950-0312
-
tuw.event.name
APMRS 2025
en
tuw.event.startdate
12-09-2025
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tuw.event.enddate
13-09-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
APMA, TU Wien
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tuw.event.presenter
Hofreither, Dominik
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Medizinische Biotechnologie
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wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
3040
-
wb.sciencebranch.value
50
-
wb.sciencebranch.value
50
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item.languageiso639-1
en
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item.grantfulltext
none
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.fulltext
no Fulltext
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
Department of Biotechnology - BOKU University (Vienna, AT)
-
crisitem.author.dept
University of Salzburg (Salzburg, AT)
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
University of Salzburg, Austria
-
crisitem.author.dept
University of Salzburg, Austria
-
crisitem.author.dept
University of Salzburg, Austria
-
crisitem.author.dept
University of Salzburg, Austria
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
Department of Biotechnology - Universität für Bodenkultur Wien (Wien, AT)
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.orcid
0009-0006-2194-706X
-
crisitem.author.orcid
0009-0004-5578-3628
-
crisitem.author.orcid
0000-0001-8076-3187
-
crisitem.author.orcid
0000-0003-4025-9968
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.orcid
0000-0001-6324-9338
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
