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<div class="csl-entry">Saghafi, S., Foroughipour, M., Foroughipour, M., Oakes, J., & Becker, K. (2025, June 9). <i>Designing a tunable light sheet generator with enhanced optical characteristics</i> [Conference Presentation]. Photonics Prague 2025-9th International Conference on Photonics, Devices and Systems, Prague, Czechia. http://hdl.handle.net/20.500.12708/219783</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/219783
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dc.description
This work presents the simulation, optical design, and assembly of a tunable light sheet microscope for high-resolution 3D imaging of biological specimens. The system uses aspheric lenses combined with a single Powell lens, which induces a phase shift along one direction, producing a bone-shaped beam that minimizes the central hot spot and creates a semi-uniform intensity distribution. Simulations and experiments show that a thin light sheet can be achieved by combining a Powell lens with other aspherical elements. Optimal results were obtained with Powell lenses featuring fan angles of 5° to 10° and tip radii of 0.1 mm to 0.4 mm. The system was validated by imaging biological specimens of various sizes, demonstrating enhanced resolution along all spatial axes. This improved imaging performance highlights the system’s potential for high-resolution tissue and neural/vascular network imaging in basic research and medical applications.
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dc.description.abstract
In this work we describe simulation, optical design and assembly of a tunable light sheet microscope capable of high-resolution 3D imaging of biological specimen. The system utilizes aspheric lenses that are combined with one axial-conical lens (e.g. Powell lens). Due to the conic aspheric shape of such lenses a special phase shift transformation is induced along one direction if an incident laser beam interacts with the tip of the lens. Unlike conventional laser line generators, such as cylindrical lenses, Powell lenses redirect the beam towards their edges forming a bone-shaped beam. This effectively minimizes the central hot spot and enables the formation of a semi-uniform intensity distribution at the center of the output beam. Our simulated and experimental results demonstrate that a thin light sheet can be achieved with a combination of a single Powell lens and further aspherical optical elements. We obtained best results using Powell lenses providing fan angles between 5° and 10° and tip-radii between 0.1 mm and 0.4 mm. To validate this optical design, we imaged various biological specimen in sizes ranging from several hundred µm up to multiple centimeters. The captured images reveal marked improvements in resolution along all spatial axes.
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dc.description.sponsorship
WWTF Wiener Wissenschafts-, Forschu und Technologiefonds
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dc.language.iso
en
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dc.subject
Laser beam shaping
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dc.subject
Light sheet microscopy
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dc.subject
Meso-aspheric Optics
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dc.title
Designing a tunable light sheet generator with enhanced optical characteristics
