<div class="csl-bib-body">
<div class="csl-entry">Holub, E., Hondl, N., Wöhrer, S., Lendl, B., & Ramer, G. (2025). Not Just Better Resolution: A Detailed Study of the Signal Distribution in Mid-Infrared Optical Photothermal Imaging. <i>Analytical Chemistry</i>, <i>97</i>(39), 21418–21427. https://doi.org/10.1021/acs.analchem.5c03194</div>
</div>
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dc.identifier.issn
0003-2700
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/219944
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dc.description.abstract
The spatial resolution of an imaging system is generally evaluated by analysis of the point spread function (PSF), that is, the system response to a point source in the object plane. The PSF, albeit a fundamental concept in light microscopy, has been sparsely studied in optical photothermal infrared (O-PTIR) imaging. Although there is only a minor difference between the photothermal and optical intensity distributions in the radial direction, the axial photothermal signal is markedly different from the imaging response of conventional light microscopes. Previous studies of particles beyond the visible diffraction limit have shown that the O-PTIR signal has two lobes and strongly varies with the offset between the foci of the pump and the probe laser. We will therefore present theoretical and experimental data to investigate the radial and axial intensity distributions of a commercial Raman microscope and a custom O-PTIR transmission instrument, with particular emphasis on the axial PSF of the O-PTIR system. Our data suggest that the two-lobed signal shape is also present in objects on the order of the probe beam wavelength.
en
dc.description.sponsorship
European Commission
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dc.description.sponsorship
Christian Doppler Forschungsgesells
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dc.language.iso
en
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dc.publisher
AMER CHEMICAL SOC
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dc.relation.ispartof
Analytical Chemistry
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dc.subject
photothermal spectroscopy
en
dc.subject
vibrational spectroscopy
en
dc.subject
chemical imaging
en
dc.title
Not Just Better Resolution: A Detailed Study of the Signal Distribution in Mid-Infrared Optical Photothermal Imaging
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dc.type
Article
en
dc.type
Artikel
de
dc.identifier.pmid
40982653
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dc.description.startpage
21418
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dc.description.endpage
21427
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dc.relation.grantno
953234
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dc.relation.grantno
Fortgeschrittene MIR Laserspektroskopie
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dc.type.category
Original Research Article
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tuw.container.volume
97
-
tuw.container.issue
39
-
tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.project.title
Tumor und Lymphknoten auf einer Chip Plattform für Krebsstudien
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tuw.project.title
Christian Doppler Labor für Fortgeschrittene MIR Laserspektroskopie in der (Bio-)prozessanalytik
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tuw.researchTopic.id
C6
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tuw.researchTopic.name
Modeling and Simulation
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tuw.researchTopic.value
100
-
dcterms.isPartOf.title
Analytical Chemistry
-
tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
-
tuw.publisher.doi
10.1021/acs.analchem.5c03194
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dc.date.onlinefirst
2025-09-22
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dc.identifier.eissn
1520-6882
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dc.description.numberOfPages
10
-
tuw.author.orcid
0009-0006-0703-9524
-
tuw.author.orcid
0009-0001-9282-4474
-
tuw.author.orcid
0009-0006-9836-118X
-
tuw.author.orcid
0000-0003-3838-5842
-
tuw.author.orcid
0000-0001-8307-5435
-
dc.description.sponsorshipexternal
FWF
-
dc.description.sponsorshipexternal
FWF
-
dc.relation.grantnoexternal
10.55776/COE7
-
dc.relation.grantnoexternal
10.55776/DOC142
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wb.sci
true
-
wb.sciencebranch
Chemie
-
wb.sciencebranch
Physik, Astronomie
-
wb.sciencebranch.oefos
1040
-
wb.sciencebranch.oefos
1030
-
wb.sciencebranch.value
50
-
wb.sciencebranch.value
50
-
item.languageiso639-1
en
-
item.grantfulltext
none
-
item.openairetype
research article
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
-
item.cerifentitytype
Publications
-
item.fulltext
no Fulltext
-
crisitem.project.funder
European Commission
-
crisitem.project.funder
Christian Doppler Forschungsgesells
-
crisitem.project.grantno
953234
-
crisitem.project.grantno
Fortgeschrittene MIR Laserspektroskopie
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.orcid
0009-0006-0703-9524
-
crisitem.author.orcid
0009-0001-9282-4474
-
crisitem.author.orcid
0009-0006-9836-118X
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren