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<div class="csl-entry">Saghafi, S., Foroughipour, S. M., Foroughipour, M., Prado-López, S., Oakes, J., & Becker, K. (2025). Beam width tunability using refractive optics in static light sheet microscopy. In K. Fliegel & V. Prajzler (Eds.), <i>Photonics, Devices, and Systems IX : Proceedings of SPIE</i>. https://doi.org/10.1117/12.3072732</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/220005
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dc.description.abstract
This study presents a tunable light sheet microscope for high-resolution three-dimensional biological imaging. The core innovation integrates aspheric lenses with a single Powell lens in the optical pathway. The Powell lens introduces a phase shift that creates a bone-shaped beam profile, reducing the central intensity hot spot and achieving more uniform intensity distribution. Simulations and experimental results confirm this optical configuration generates thin, high-quality light sheets. Optimal performance occurs with Powell lenses having fan angles of 5°-10° and tip radii of 0.1-0.4 mm. The system demonstrates advanced imaging capabilities through visualization of various biological specimens. This establishes the microscope as a powerful tool for high-resolution studies of tissues, neural networks, and vascular systems in fundamental research and medical applications.
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dc.description.sponsorship
WWTF Wiener Wissenschafts-, Forschu und Technologiefonds
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dc.description.sponsorship
Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftu
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dc.language.iso
en
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dc.subject
Static light sheet microscopy
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dc.subject
beam shaping
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dc.subject
cylindrical lenses
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dc.subject
refractive optics
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dc.subject
tunable beam width
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dc.subject
optical design
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dc.title
Beam width tunability using refractive optics in static light sheet microscopy