Neubauer, Lena

Failla, Martina

DOSS, Jérôme

Andrieu, Guillaume

Lendl, Bernhard

Ramer, Georg

Deniset-Besseau, Ariane

2025-10-20T09:21:02Z

2025-10-20T09:21:02Z

2025-02-02

<div class="csl-bib-body"> <div class="csl-entry">Neubauer, L., Failla, M., DOSS, J., Andrieu, G., Lendl, B., Ramer, G., &#38; Deniset-Besseau, A. (2025, February 2). <i>Investigation at the nanoscale of membrane lipids peroxidation  in T-cells using AFM-IR</i> [Poster Presentation]. XXIV. Linz Winter Workshop 2025, Linz, Austria. http://hdl.handle.net/20.500.12708/220123</div> </div>

http://hdl.handle.net/20.500.12708/220123

T-cell acute lymphoblastic leukaemia (T-ALL) is a malignant haematological cancer which poses significant therapeutic challenges due to a high level of refractory and relapsed cases and an overall low survival rate (< 50% in adults).1,2 Ferroptosis, a regulated, non-apoptotic cell death mechanism driven by iron-mediated lipid peroxidation in cells’ membranes, has emerged as a potential therapeutic strategy for T-ALL. Investigations show that specific T-cell types have impaired regulation of radical oxygen species (ROS) production, making them more susceptible to ferroptosis. This mechanism could be harnessed by artificially increasing ROS levels to target and eliminate refractory T-cells. However, the direct impact of ferroptosis inducers on the cell membranes is not fully understood and has never been characterized at nanoscale.3 To explore cells’ membrane ferroptosis-induced modifications, novel analytical techniques enabling chemical characterization at the nanoscale are required. Thus, we propose to use atomic force microscopy coupled with infrared (IR) spectroscopy (AFM-IR)4 - a technique allowing chemical identification with a spatial resolution of about 20 nm – to track the membrane modifications induced by peroxidation processes. A robust and reproducible AFM-IR protocol (sample preparation and AFM-IR settings) was developed to detect and image lipid peroxidation at the nanoscale in two T-ALL cell lines, Loucy and Jurkat, exposed to two ferroptosis inducers called RSL3 and APR-246. As a result of high-resolution AFM-IR chemical imaging, peroxidized phospholipid hotspots were identified on cells’ plasma membranes, while local spectra at specific positions exhibited IR absorption bands associated with different oxidized phospholipid end products. Jurkat cells responded strongly to ferroptosis induction, with lipid peroxidation primarily localized in the plasma membrane and pseudopodia regions. In contrast, Loucy cells exhibited a less pronounced lipid peroxidation response. This work highlights the potential of AFM-IR to investigate cells’ membrane biochemical degradation during ferroptosis in T-ALL. A better understanding of the degradation processes provides valuable insights into ferroptosis mechanisms and could contribute to a better perception of future therapeutic strategies targeting lipid peroxidation in cancer cells. (1) Raetz, E. A. and Teachey D. T., Hematology 2016 (2) Cordo’, V. et al., Blood Cancer Discov 2021 (3) Jiang, X. et al. Nat Rev Mol Cell Biol 2021 (4) Dazzi, A. et al., Journal of Applied Physics 2010

European Commission

Christian Doppler Forschungsgesells

en

AFM-IR

nanoscale imaging

infrared spectroscopy

ferroptosis

phospholipid peroxidation

Investigation at the nanoscale of membrane lipids peroxidation in T-cells using AFM-IR

Presentation

Vortrag

Medicina e Chirurgia - University of Milano-Bicocca (Milan, IT)

Immunologie, Infectiologie et Hématologie (I2H) - Institut Necker Enfants Malades (Paris, FR)

Laboratory of Onco-Hematology - Institut Necker-Enfants Malades (Paris, FR)

953234

Fortgeschrittene MIR Laserspektroskopie

Poster Presentation

Tumor und Lymphknoten auf einer Chip Plattform für Krebsstudien

Christian Doppler Labor für Fortgeschrittene MIR Laserspektroskopie in der (Bio-)prozessanalytik

M2

M6

Materials Characterization

Biological and Bioactive Materials

60

40

E164-02-1 - Forschungsgruppe Prozessanalytik

E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials

E056-12 - Fachbereich ENROL DP

0009-0002-9359-1841

0009-0007-6883-4887

0009-0009-2705-2760

0000-0003-0289-4952

0000-0003-3838-5842

0000-0001-8307-5435

0000-0002-1923-4988

XXIV. Linz Winter Workshop 2025

31-01-2025

03-02-2025

On Site

Event for scientific audience

Linz

AT

JKU Linz - Abteilung für Angewandte Experimentelle Biophysik

Neubauer, Lena

Single Track

Chemie

Nanotechnologie

Biologie

1040

2100

1060

50

40

10

no Fulltext

en

http://purl.org/coar/resource_type/c_18co

Publications

restricted

conference poster not in proceedings

European Commission

Christian Doppler Forschungsgesells

953234

Fortgeschrittene MIR Laserspektroskopie

E164-02-1 - Forschungsgruppe Prozessanalytik

Medicina e Chirurgia - University of Milano-Bicocca (Milan, IT)

Immunologie, Infectiologie et Hématologie (I2H) - Institut Necker Enfants Malades (Paris, FR)

Laboratory of Onco-Hematology - Institut Necker-Enfants Malades (Paris, FR)

E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren

E164-02-1 - Forschungsgruppe Prozessanalytik

0009-0002-9359-1841

0009-0007-6883-4887

0009-0009-2705-2760

0000-0003-0289-4952

0000-0003-3838-5842

0000-0001-8307-5435

0000-0002-1923-4988

E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren

E164 - Institut für Chemische Technologien und Analytik

E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren