<div class="csl-bib-body">
<div class="csl-entry">Kittler, S., Müller, F., Elshazly, M., Wandrey, G. B., Klein, T., Daub, A., Spadiut, O., & Kopp, J. (2025). Transferability of bioprocessing modes for recombinant protease production: from fed-batch to continuous cultivation with Bacillus licheniformis. <i>BMC Biotechnology</i>, <i>25</i>, Article 13. https://doi.org/10.1186/s12896-025-00947-9</div>
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dc.identifier.issn
1472-6750
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/221641
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dc.description.abstract
Proteases are essential in various industries due to their unique substrate specificities and robustness in different operational conditions. Bacillus strains consist of a genotype favorable for rapid growth whilst secreting enzymes extracellularly, thereby simplifying recombinant protease production. Despite the widespread use of batch and fed-batch fermentations for their ease and robustness, these cultivation types are often marred by significant energy requirements and prolonged downtimes. The switch towards continuous cultivation methods promises reduced carbon footprints and improved equipment efficiency. Yet, research focusing on Bacillus strains is limited, therefore we aimed to establish a continuous cultivation as a competitive alternative to fed-batch.
en
dc.language.iso
en
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dc.publisher
BMC
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dc.relation.ispartof
BMC Biotechnology
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dc.subject
Bioreactors
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dc.subject
Fermentation
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dc.subject
Bacillus licheniformis
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dc.subject
Chemostat
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dc.subject
Continuous processing
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dc.subject
Fed-batch
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dc.subject
Model-based development
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dc.subject
Sustainable processing
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dc.subject
Recombinant Proteins
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dc.subject
Batch Cell Culture Techniques
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dc.subject
Peptide Hydrolases
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dc.subject
Bacterial Proteins
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dc.title
Transferability of bioprocessing modes for recombinant protease production: from fed-batch to continuous cultivation with Bacillus licheniformis
