<div class="csl-bib-body">
<div class="csl-entry">Kopp, J., Pekarsky, A., & Spadiut, O. (2025). Adapting Komagataella phaffii Cultivation Media to REACH Regulations. In X. Garcia-Ortega, A. Glieder, K. Kovar, & L. Rieder (Eds.), <i>Pichia pastoris : Methods and Protocols</i> (Vol. 2697, pp. 445–455). Humana. https://doi.org/10.1007/978-1-0716-4779-0_30</div>
</div>
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/223334
-
dc.description.abstract
REACH stands for "Registration, Evaluation, Authorization, and Restriction of Chemicals" and classifies certain substances as harmful to human health and the environment. Boric acid and cobalt dichloride, commonly used as boron (B) and cobalt (Co) sources in cultivation media for Komagataella phaffii, represent such harmful substances. However, B and Co support cellular growth, and yeast harbors a large number of co-containing enzymes. Even though cell physiology (up to 90 g/L) was not affected by the removal of boric acid and cobalt dichloride from the media, recombinant protein production varied when excluding these components from the cultivation media to comply with REACH regulations. Here, we describe how to analyze and potentially adapt a defined cultivation medium for K. phaffii bioreactor cultivations concerning current REACH regulations.
en
dc.language.iso
en
-
dc.subject
Bioreactors
en
dc.subject
Cobalt
en
dc.subject
Boric Acids
en
dc.subject
Recombinant Proteins
en
dc.subject
Boron
en
dc.subject
Boron leakage
en
dc.subject
Cobalt
en
dc.subject
Fermentation
en
dc.subject
Glass vessel
en
dc.subject
Komagataella phaffii
en
dc.subject
Pichia pastoris
en
dc.subject
REACH
en
dc.subject
Stainless steel
en
dc.subject
boron
en
dc.subject
Culture Media
en
dc.subject
Saccharomycetales
en
dc.title
Adapting Komagataella phaffii Cultivation Media to REACH Regulations
