<div class="csl-bib-body">
<div class="csl-entry">Riedlsperger, L., Lendl, B., Ramer, G., & Schwaighofer, A. (2025, April 1). <i>Calibration of Mid-IR Sensors for Complex Samples</i> [Poster Presentation]. 6th FemChem Scientific Workshop 2025, Wien, Austria. http://hdl.handle.net/20.500.12708/223495</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/223495
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dc.description.abstract
Improved process analytical technology (PAT) is required in the pharmaceutical industry to enable real-time monitoring and control of bio-pharmaceutical manufacturing processes. A particular challenge is PAT in continuous processing. Among the various PAT tools, mid-infrared (Mid-IR) spectroscopy stands out for its ability to provide rapid, molecular specific non-destructive analysis of chemical compositions.
Our research focuses on the monitoring of protein aggregation, a critical quality attribute in bio-pharmaceutical production of monoclonal antibodies. During the manufacturing and purification of monoclonal antibodies, the high background absorbance of aqueous solutions makes in-line transmission measurements with traditional Fourier transform infrared (FTIR) spectrometers unfeasible. With the use of a quantum cascade laser, a tuneable, high spectral power density mid-IR source, flow-through absorbance measurements of the amide I and amide II band regions of proteins are attainable. However, important issues remain: Matrix changes (e.g. buffer, pH, temperature changes) can lead to absorbance bands that overlap or distort the protein bands. This requires the use of multivariate data analysis and large calibration data sets. Due to the uncertain purity of commercial proteins and the resulting variability in the final concentration of calibration solutions, reference measurements are critical. This research aims to address the difficulties in calibrating a mid-IR spectroscopic sensor for complex samples, with a particular focus on its application in the purification of monoclonal antibodies.
en
dc.description.sponsorship
Christian Doppler Forschungsgesells
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dc.language.iso
en
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dc.subject
Protein Analysis
en
dc.subject
Process analytical technology (PAT)
en
dc.subject
Mid-IR Laser Spectroscopy
en
dc.title
Calibration of Mid-IR Sensors for Complex Samples
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
Fortgeschrittene MIR Laserspektroskopie
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dc.type.category
Poster Presentation
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tuw.project.title
Christian Doppler Labor für Fortgeschrittene MIR Laserspektroskopie in der (Bio-)prozessanalytik
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tuw.researchTopic.id
Q1
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
C6
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tuw.researchTopic.name
Photonics
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Modeling and Simulation
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tuw.researchTopic.value
30
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tuw.researchTopic.value
20
-
tuw.researchTopic.value
50
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tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
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tuw.publication.orgunit
E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials
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tuw.publication.orgunit
E056-12 - Fachbereich ENROL DP
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tuw.author.orcid
0009-0009-7577-4397
-
tuw.author.orcid
0000-0003-3838-5842
-
tuw.author.orcid
0000-0001-8307-5435
-
tuw.author.orcid
0000-0003-2714-7056
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tuw.event.name
6th FemChem Scientific Workshop 2025
en
tuw.event.startdate
01-04-2025
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tuw.event.enddate
01-04-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Wien
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tuw.event.country
AT
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tuw.event.institution
TU Wien
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tuw.event.presenter
Riedlsperger, Lisa
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wb.sciencebranch
Chemie
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wb.sciencebranch
Physik, Astronomie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
1030
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wb.sciencebranch.value
80
-
wb.sciencebranch.value
20
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.grantfulltext
restricted
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.openairetype
conference poster not in proceedings
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crisitem.project.funder
Christian Doppler Forschungsgesells
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crisitem.project.grantno
Fortgeschrittene MIR Laserspektroskopie
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.orcid
0009-0009-7577-4397
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.orcid
0000-0003-2714-7056
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
