<div class="csl-bib-body">
<div class="csl-entry">Riedlsperger, L., Lendl, B., Ramer, G., & Dabrowska, A. (2025, May 9). <i>Multi-Pathlength Mid-IR Spectroscopy for Optimal Absorbance</i> [Conference Presentation]. Junganalytiker:innen Forum 2025, Innsbruck, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/223543
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dc.description.abstract
Mid-infrared spectroscopy is a well-established method for analysing proteins and peptides, as it tempts with its rapid, label-free and highly selective analysis. The protein spectrum, with its characteristic amid I (1600 – 1700 cm-1) and amide II (1500 – 1600 cm-1) bands, provides valuable information on both the secondary structure as well as the concentration of proteins. However, transmission measurements of aqueous solutions are challenging due to the high background absorbance of the water matrix, which leads to reduced sensitivity or even complete signal saturation.
Here, we report a novel setup that integrates a tunable quantum cascade laser (EC-QCL), a wedge-shaped transmission flow cell, and a pyroelectric array detector. This configuration enables the simultaneous measurement of liquid samples across multiple optical pathlengths, allowing for the analysis over a wide range of concentrations while remaining within the dynamic range of the detector. To determine the optimal pathlength at each wavenumber, we introduce the pathlength-to-noise ratio (PNR) as a new performance metric. PNR accounts for the influence of variable pathlengths on the detectability of a substance while ensuring low noise levels. We demonstrate the advantages of this multi-pathlength approach by measuring aqueous solutions of bovine serum albumin (BSA) and beta-lactoglobulin (bLG). While this work serves as a successful proof-of-concept, several challenges remain. The potential for future refinements will be discussed in detail.
en
dc.description.sponsorship
Christian Doppler Forschungsgesells
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dc.language.iso
en
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dc.subject
Mid-IR Laser Spectroscopy
en
dc.subject
Multi-pathlength transmission spectroscopy
en
dc.subject
Pathlength-to-noise ratio
en
dc.subject
Protein Analysis
en
dc.title
Multi-Pathlength Mid-IR Spectroscopy for Optimal Absorbance
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
Fortgeschrittene MIR Laserspektroskopie
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dc.type.category
Conference Presentation
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tuw.project.title
Christian Doppler Labor für Fortgeschrittene MIR Laserspektroskopie in der (Bio-)prozessanalytik
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tuw.researchTopic.id
Q1
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tuw.researchTopic.id
C6
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Photonics
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tuw.researchTopic.name
Modeling and Simulation
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tuw.researchTopic.name
Beyond TUW-research focus
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tuw.researchTopic.value
50
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tuw.researchTopic.value
10
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tuw.researchTopic.value
40
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tuw.publication.orgunit
E164-02-1 - Forschungsgruppe Prozessanalytik
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tuw.publication.orgunit
E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials
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tuw.publication.orgunit
E056-12 - Fachbereich ENROL DP
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tuw.author.orcid
0009-0009-7577-4397
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tuw.author.orcid
0000-0003-3838-5842
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tuw.author.orcid
0000-0001-8307-5435
-
tuw.author.orcid
0000-0002-3074-5674
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tuw.event.name
Junganalytiker:innen Forum 2025
de
tuw.event.startdate
08-05-2025
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tuw.event.enddate
09-05-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Innsbruck
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tuw.event.country
AT
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tuw.event.institution
ASAC
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tuw.event.presenter
Riedlsperger, Lisa
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.languageiso639-1
en
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item.grantfulltext
none
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item.fulltext
no Fulltext
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crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
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crisitem.author.dept
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
-
crisitem.author.dept
E164-02-1 - Forschungsgruppe Prozessanalytik
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crisitem.author.orcid
0009-0009-7577-4397
-
crisitem.author.orcid
0000-0003-3838-5842
-
crisitem.author.orcid
0000-0001-8307-5435
-
crisitem.author.orcid
0000-0002-3074-5674
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
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crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
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crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
-
crisitem.author.parentorg
E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren
