Krauß, Judith Elisabeth Margareta

Leuschner, Chiara

Wöhrer, Sebastian

Lendl, Bernhard

Kittler, Stefan

Rausch, Clemens

Spadiut, Oliver

Ertl, Peter

Schobesberger, Silvia

Frauenlob, Martin

2026-01-13T08:32:56Z

2026-01-13T08:32:56Z

2025-06-11

<div class="csl-bib-body"> <div class="csl-entry">Krauß, J. E. M., Leuschner, C., Wöhrer, S., Lendl, B., Kittler, S., Rausch, C., Spadiut, O., Ertl, P., Schobesberger, S., &#38; Frauenlob, M. (2025, June 11). <i>Investigation on compound leaching of DLP 3D printed materials to advance microfluidic cell culture</i> [Poster Presentation]. MPS World Summit 2025, Brussels, Belgium. http://hdl.handle.net/20.500.12708/224066</div> </div>

http://hdl.handle.net/20.500.12708/224066

Among other 3D printing methods DLP printing has gained signifi cant interest for rapid prototyping of lab- and organ-on-a-chip devic es, because of its fabrication speed and high resolution. Compared to conventional chip fabrication methods it streamlines the process from design to finished product, eliminating labor-intensive steps such as photolithography, casting, and bonding [1]. Despite its benefits, the resin materials used in DLP printing rely mainly on radical photo-initiator-based polymerization, which cannot achieve complete polymerization, particularly in bulk materials. Cy totoxic monomers, crosslinkers and photo initiators may remain in the material and leach out over time, negatively affecting the microphysi ological systems [2]. Therefore, we aim to develop a streamlined process to rapidly as sess the suitability of resins for use in microfluidic cell culture. The first step involves identifying the optimal post-processing condition through the evaluation of (1) the degree of curing using Raman spec troscopy on both the surface and inside the bulk material, and (2) the leaching of potentially cytotoxic components into cell culture medium via HPLC. The second phase assesses material compatibility on a bio logical level by performing a 24-hour cell viability assay with human fibroblasts comparing the optimal condition to the uncured material as proof of concept. Finally, the optimized parameters are applied in long-term on-chip studies with dynamic cell culture to evaluate cell stress, adhesion, and leaching via PCR markers and HPLC. Unlike previous studies, this approach emphasizes the long-term evaluation of leaching, coupled with molecular-biological assessments [1]. First results show that polymerization efficiency is influenced by an interplay of material thickness, washing, UV and heat treatment steps which all affect leaching of compounds. Preliminary cell studies in dicate viable fibroblast cultures with optimized post-processing over five days. In conclusion, this approach highlights the necessity to evaluate DLP-printed materials for microfluidic cell culture as fabrication pa rameters need optimization to minimize adverse effects on MPS. The process could also be adapted for assessing material reusability, pro viding a sustainable method for rapid prototyping. References [1] Fritschen, A., Bell, A., Königstein, I. et al. (2022). Biomater Sci 8, 1981-1994. doi:10.1039/D1BM01794B [2] Bagheri, A. and Jin, J. (2019). ACS Appl Polym Mater 1, 593-611. doi:10.1021/acsapm.8b00165 Presentation: Poster

en

3D printing

Microfluidics

leaching

cytocompatability

Investigation on compound leaching of DLP 3D printed materials to advance microfluidic cell culture

Presentation

Vortrag

Poster Presentation

M7

M2

M1

Special and Engineering Materials

Materials Characterization

Surfaces and Interfaces

20

60

20

https://proceedings.altex.org/data/2025_01/altex_MPS2025.pdf (page 114)

E163-03-1 - Forschungsgruppe Cell Chip

E164-02-3 - Forschungsgruppe Cell Chip

E056-04 - Fachbereich TU-DX: Towards Applications of 2D Materials

E056-12 - Fachbereich ENROL DP

E057-10 - Fachbereich Gruppe Angepasste Technologie

E166-04-1 - Forschungsgruppe Bioprozess-Technologie

E166-04-2 - Forschungsgruppe Integrierte Bioprozessentwicklung

0009-0006-5312-2986

0009-0006-9836-118X

0000-0003-3838-5842

0009-0006-5989-1209

0000-0001-7460-9378

MPS World Summit 2025

09-06-2025

13-06-2025

On Site

Event for scientific audience

Brussels

BE

Krauß, Judith Elisabeth Margareta

Chemie

Chemische Verfahrenstechnik

Pharmazie, Pharmakologie, Toxikologie

1040

2040

3012

60

20

20

conference poster not in proceedings

http://purl.org/coar/resource_type/c_18co

Publications

en

none

no Fulltext

E163-03-1 - Forschungsgruppe Cell Chip

E163-03-1 - Forschungsgruppe Cell Chip

E164-02-1 - Forschungsgruppe Prozessanalytik

E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren

E166-04-2 - Forschungsgruppe Integrierte Bioprozessentwicklung

E166-04-2 - Forschungsgruppe Integrierte Bioprozessentwicklung

E166-04 - Forschungsbereich Bioverfahrenstechnik

E610 - Vizerektorat Forschung und Innovation

E163-03-1 - Forschungsgruppe Cell Chip

E163-03-1 - Forschungsgruppe Cell Chip

0009-0006-5312-2986

0009-0006-9836-118X

0000-0003-3838-5842

0009-0006-5989-1209

0000-0003-0916-0644

0000-0002-7625-2445

0000-0001-7460-9378

E163-03 - Forschungsbereich Organische und Biologische Chemie

E164-02 - Forschungsbereich Umwelt-, Prozessanalytik und Sensoren

E164 - Institut für Chemische Technologien und Analytik

E166-04 - Forschungsbereich Bioverfahrenstechnik

E166-04 - Forschungsbereich Bioverfahrenstechnik

E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften

E000 - Technische Universität Wien

E163-03 - Forschungsbereich Organische und Biologische Chemie

E163-03 - Forschungsbereich Organische und Biologische Chemie