<div class="csl-bib-body">
<div class="csl-entry">Krošelj, S., Mikulic-Petkovsek, M., Likar, M., Škvarč, A., Halbwirth, H., Biniari, K., & Rusjan, D. (2025). The Status of Esca Disease and the Disinfection of the Scion Prior to Grafting Affect the Phenolic Composition and Phenylpropanoid-Related Enzymes in the Callus of Vine Hetero-Grafts. <i>Horticulturae</i>, <i>11</i>(4), Article 371. https://doi.org/10.3390/horticulturae11040371</div>
</div>
-
dc.identifier.issn
2311-7524
-
dc.identifier.uri
http://hdl.handle.net/20.500.12708/224092
-
dc.description.abstract
Vegetative propagation of European grapevine (Vitis vinifera L.) requires grafting onto American rootstocks due to susceptibility to phylloxera. However, the grafting yield is compromised by the presence of grapevine trunk diseases (GTDs) such as Esca. This study investigates the phenolic response and enzyme activity in grapevine callus from grafts obtained by scions with different GTD status (healthy, asymptomatic, and symptomatic) treated with different disinfection methods (Beltanol, Beltanol in combination with thermotherapy, Serenade® ASO, Remedier, BioAction ES, and sodium bicarbonate). Twenty-three phenolic compounds were identified in the graft callus, with flavanols, stilbenes, and condensed tannins predominating. Scion disinfection with BioAction ES led to a significant increase in total phenolic content in the callus, especially in symptomatic scions, for on average 510.3 µg/g fresh weight (FW) higher total phenolic content, compared to grafts where scions were treated with Beltanol. Phenolics such as epicatechin gallate, procyanidin derivatives, and resveratrol hexoside were significantly increased, indicating a strong elicitor effect of BioAction ES. Enzymatic activity analysis showed that the disinfection methods affected the activity of key enzymes involved in the phenylpropanoid metabolic pathway. In particular, BioAction ES significantly increased phenylalanine ammonia lyase (PAL) activity in callus from grafts with healthy scions by 3.4-fold and flavanone 3β-hydroxylase (FHT) activity in callus from grafts with infected scions by 4.9-fold (asymptomatic) and 6.9-fold (symptomatic) compared to callus from grafts with Beltanol-treated scions. The results highlight the potential of environmentally friendly disinfection methods, particularly BioAction ES, in influencing phenolic content and enzymatic activity in graft callus, potentially affecting the success of grapevine grafting.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
-
dc.language.iso
en
-
dc.publisher
MDPI
-
dc.relation.ispartof
Horticulturae
-
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
-
dc.subject
disinfection
en
dc.subject
grafting
en
dc.subject
grapevine trunk diseases
en
dc.subject
nursery
en
dc.subject
phenolic compounds
en
dc.title
The Status of Esca Disease and the Disinfection of the Scion Prior to Grafting Affect the Phenolic Composition and Phenylpropanoid-Related Enzymes in the Callus of Vine Hetero-Grafts