<div class="csl-bib-body">
<div class="csl-entry">Fernández-Pérez, J. (2025, September 26). <i>Exploring light-based biofabrication of norbornene-functionalized polymers for recreation of cell microenvironments</i> [Keynote Presentation]. 15th International Symposium in Biomedical Polymers, Porto, Portugal.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/224635
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dc.description.abstract
This talk will describe two polymer systems which have been functionalized with norbornene groups to enable hydrogel formation via thiol-ene chemistry. For both systems, an all-inone pot approach was used to form the hydrogels in cell-permissive conditions. Peptide sequences cleavable by cell-secreted matrix metalloproteinases flanked by cysteines were used as cross-linkers. Polymers were rendered cell adhesive by binding of cysteineterminated RGD sequences. Cell were included to the mix. LAP was used as photoinitiator, and pre-gel solution exposed to 30 seconds of UV light to induce cross-linking.
The first system is based on naturally-derived polysaccharide alginate. By tuning polymer and cross-linker concentrations a stiffness range between 300 to 2100 Pa was achieved. Full cross-linking occured at 12 seconds post UV irradiation, as determined by photorheometry. Scanning electron microscopy showed a highly porous ultrastructure. Hydrogels degraded upon exposure to exogenous collagenase. Pre-gel solutions could be successfully bioprinted with a pneumatic extrusion-based system. A variety of delicate cell types were encapsulated in the hydrogels. Human endometrial organoids presented high cell viability, grew in size over time, presented spherical morphology, and expressed cell-cell contacts E-cadherin and proliferation marker ki67. Encapsulated mouse embryonic stem cell-derived thyroid follicles produced thyroglobulin and T4. Mouse intestinal organoids adopted a proliferative phenotype. Vascularization inside the hydrogels was achieved using endothelial cells and
supporting cells (single cell suspension and spheroids). Neurite outgrowth, both small and thick bundles, from encapsulated iPSC-derived neurospheres demonstrated the hydrogel’s reinnervation potential.
The second system is based on synthetic polyvinyl alcohol (PVA). PVA is water soluble, biocompatible, has good mechanical properties and is highly amenable to chemical
modifications. At 5% w/v hydrogels had a stiffness of approximately 2000 Pa. Exogenous collagenase degraded hydrogels cross-linked with MMP, but not when PEG was used. Dermal fibroblasts were encapsulated and presented elongated and spread morphology. Granular hydrogels were obtained upon secondary cross-linking of packed hydrogel particles formed by extrusion fragmentation and by emulsion in silicon oil. Fibroblasts could attach onto the surface of the hydrogel particles and migrate through the microporous structure. Future work will focus on studying the influence of inter-particle crosslinking on cell behavior, and the biofabrication of hierarchical constructs using granular hydrogels.
These two polymer platforms allow high tunability of mechanical and biological properties to tailor the needs of cell culture, which could be an alternative to basement-membrane extracts.
en
dc.language.iso
en
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dc.subject
Polymers
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dc.subject
Biofabrication
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dc.subject
tissue engineering
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dc.title
Exploring light-based biofabrication of norbornene-functionalized polymers for recreation of cell microenvironments
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dc.type
Presentation
en
dc.type
Vortrag
de
dc.type.category
Keynote Presentation
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tuw.publication.invited
invited
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tuw.researchTopic.id
M6
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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tuw.author.orcid
0000-0002-8375-2907
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tuw.event.name
15th International Symposium in Biomedical Polymers
en
tuw.event.startdate
22-09-2025
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tuw.event.enddate
26-09-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Porto
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tuw.event.country
PT
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tuw.event.presenter
Fernández-Pérez, Julia
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch
Biologie
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wb.sciencebranch
Werkstofftechnik
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
2050
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wb.sciencebranch.value
30
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wb.sciencebranch.value
20
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wb.sciencebranch.value
50
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.languageiso639-1
en
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item.grantfulltext
none
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item.fulltext
no Fulltext
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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crisitem.author.orcid
0000-0002-8375-2907
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crisitem.author.parentorg
E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
