<div class="csl-bib-body">
<div class="csl-entry">Aurum, F. S., Julian, R., Prantl, R., Giada, M., Schröder, K., & Pignitter, M. (2025, October 12). <i>Upcycling Coffee Waste: Sustainable Oil Recovery and Lipidomic Profiling of Spent Coffee Grounds</i> [Conference Presentation]. 20th Euro Fed Lipid Congress and Expo, Leipzig, Germany. http://hdl.handle.net/20.500.12708/224945</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/224945
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dc.description.abstract
Spent coffee grounds (SCG), a major by-product of the global coffee industry, represent an underexploited source of bioactive lipids with promising applications in cosmetics, nutraceuticals, and functional foods. This study presents a novel, integrative approach that combines green extraction techniques with advanced multi-omics profiling to sustainably recover and characterize high-value oil from SCG.
Three green extraction methods, ultrasonic-assisted extraction (UAE), supercritical CO₂ (SC-CO₂), and ethanol maceration, were evaluated alongside conventional Soxhlet hexane extraction. The oil yield ranged from 8.3% (SC-CO2) to 10.5% (UAE), with green techniques demonstrating superior efficiency and compound preservation. Untargeted lipidomics was conducted using LC-QTOF-MS with reversed-phase chromatography to comprehensively profile triacylglycerols, ceramides, Fatty Acid esters of Hydroxy Fatty Acids (FAHFAs), and oxidized lipids. Targeted LC-MS/MS quantified oxidized phosphatidylcholines (OxPCs), while HPLC was used to identify and quantify α-, β-, and γ-tocopherols. Volatile compounds of the extracted oil were analyzed via HS-SPME GC-MS, and polar metabolites were profiled using HILIC-LC-QTOF-MS.
Lipidomic data were processed using open-source tools, including MS-DIAL and GNPS, allowing for robust data processing, deconvolution, alignment and identification. Multivariate analysis of PLS-DA models showed clear separation and tight clustering of samples according to extraction method, reflecting distinct lipidomic signatures. Importantly, green extraction techniques led to a 50% increase in FAHFAs and over 80% higher ceramide content compared to Soxhlet extracts. These findings reveal not only greater lipid diversity but also enhanced recovery of functionally important compounds known for anti-inflammatory, skin health, and metabolic benefits.
In contrast, green extraction techniques resulted in a markedly lower contet of OxPC species, which are often associated with lipid degradation and oxidative stress. This suggests that UAE, SC-CO₂, and ethanol maceration provide a milder extraction environment, minimizing lipid oxidation compared to Soxhlet hexane extraction. Additionally, tocopherol content—particularly α-, β-, and γ-tocopherols—was significantly higher in green extracts, indicating better preservation of natural antioxidants.
This work demonstrates the synergistic power of green chemistry and omics-driven analysis for the valorization of agri-food waste. The comprehensive dataset and methodological framework provide a scalable model for transforming SCG into high-value bioactive ingredients, contributing the way for more sustainable and economically viable industrial applications.
en
dc.language.iso
en
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dc.subject
Biomass valorization
en
dc.subject
Spent Coffee Grounds (SCG)
en
dc.subject
Supercritical CO2 extraction
en
dc.subject
Lipidomics
en
dc.title
Upcycling Coffee Waste: Sustainable Oil Recovery and Lipidomic Profiling of Spent Coffee Grounds
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
University of Vienna, Austria
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dc.contributor.affiliation
University of Vienna, Austria
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dc.contributor.affiliation
University of Vienna, Austria
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dc.contributor.affiliation
University of Vienna, Austria
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dc.type.category
Conference Presentation
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Beyond TUW-research focus
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E163-03-5 - Forschungsgruppe Nachhaltige organische Synthese und Katalyse
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tuw.publication.orgunit
E056-09 - Fachbereich CO2 Refinery
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tuw.author.orcid
0000-0002-2515-9873
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tuw.event.name
20th Euro Fed Lipid Congress and Expo
en
tuw.event.startdate
12-10-2025
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tuw.event.enddate
15-10-2025
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Leipzig
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tuw.event.country
DE
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tuw.event.presenter
Pignitter, M.
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wb.sciencebranch
Chemie
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wb.sciencebranch
Chemische Verfahrenstechnik
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wb.sciencebranch
Pharmazie, Pharmakologie, Toxikologie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.oefos
2040
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wb.sciencebranch.oefos
3012
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wb.sciencebranch.value
60
-
wb.sciencebranch.value
20
-
wb.sciencebranch.value
20
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item.openairetype
conference paper not in proceedings
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.cerifentitytype
Publications
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item.languageiso639-1
en
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item.grantfulltext
none
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item.fulltext
no Fulltext
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crisitem.author.dept
University of Vienna, Austria
-
crisitem.author.dept
University of Vienna, Austria
-
crisitem.author.dept
University of Vienna, Austria
-
crisitem.author.dept
E163-03-5 - Forschungsgruppe Nachhaltige organische Synthese und Katalyse
-
crisitem.author.dept
E163-03-5 - Forschungsgruppe Nachhaltige organische Synthese und Katalyse
-
crisitem.author.dept
University of Vienna, Austria
-
crisitem.author.orcid
0000-0002-2515-9873
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
