Baltzaki, Maria Chrysi Izampella

Schmidtbauer, Anna

Markovic, Marica

Slezak, Paul

Redl, Heinz

Baudis, Stefan

2026-01-20T09:07:24Z

2026-01-20T09:07:24Z

2025-05-20

<div class="csl-bib-body"> <div class="csl-entry">Baltzaki, M. C. I., Schmidtbauer, A., Markovic, M., Slezak, P., Redl, H., &#38; Baudis, S. (2025, May 20). <i>Human Platelet Lysate‑functionalized hydrogels – An innovative approach for Bone tissue regeneration</i> [Poster Presentation]. Tissue Engineering and Regenerative Medicine Society (TERMIS) European Congress 2025, Freiburg, Germany. http://hdl.handle.net/20.500.12708/224987</div> </div>

http://hdl.handle.net/20.500.12708/224987

Introduction/Objectives Critical-size bone defects cause significant challenges to bone regeneration, which can affect the healing process and recovery. Bone implants are usually employed for treating these issues. Their biointegration, however, is challenged by large defect sizes and their effect on biological processes such as the transport of vital nutrients and cell growth.[1] This study focuses on developing bio-functionalized hydrogels from modified Human Platelet Lysate (PL) for enhancing the osseointegration of 3D-printed bone implants. By leveraging the cell-instructive and regenerative properties of PL and improving its mechanical strength through photopolymerizable derivatives, existing boundaries in scaffold-assisted bone regeneration can be overcome. Methods The PL was modified with photopolymerizable groups (i.e. allyl glycidyl ether (PLAGE)) and was subsequently crosslinked under UV irradiation. The hydrogel’s properties were compared to similar gelatin-based systems. Extensive swelling tests, in situ photorheology, and in vitro studies comprised a detailed characterization of the materials. Preliminary in vivo tests of the most promising PL-based hydrogels provided insight on the material’s performance in living organisms. Results A variety of photocrosslinkable PL alternatives were synthesized and crosslinked under UV light. The crosslinking of these photopolymerizable motifs in the modified PL’s backbone resulted in improvement of the mechanical properties compared to those of unmodified PL. Furthermore, successful hydrogel crosslinking was achieved both in presence and absence of thiols. Since PL is primarily based on serum albumin, cysteine is one of its most prevalent amino acid. Therefore, the thiol groups of the protein’s backbone further enhanced gel formation in PL. The applicability of the crosslinked modified PL in Tissue Engineering and Regenerative Medicine was investigated further. A pilot in vivo test on dorsal subcutaneous mouse model was conducted for the most promising PL-based hydrogels, in order to investigate the acute response, degradation and grade of inflammation. Conclusions A promising and versatile PL-based toolbox was developed within this study including a different photopolymerizable PL modifications. Different crosslinking approaches were tested and were followed by thorough mechanical characterization. By tweaking the parameters, a promising system for 3D biofabrication can be developed to provide a novel solution for bone tissue engineering. Acknowledgements Christian Doppler Research Association, Austrian Federal Ministry for Digital & Economic Affairs, National foundation for Research, Technology & Development References 1. Nauth A., Schemitsch E, Norris B., Nollin Z., Watson J.T., J. Orthop. Trauma, 2018. 32.

Christian Doppler Forschungsgesells

en

platelet lysate

Hydrogels

Bone Tissue Engineering

Human Platelet Lysate‑functionalized hydrogels – An innovative approach for Bone tissue regeneration

Presentation

Vortrag

TU Wien, Austria

TU Wien, Austria

Ludwig Boltzmann Gesellschaft, Austria

CDL Baudis

Poster Presentation

Christian Doppler Labor für Fortschrittliche Polymere für Biomaterialien und den 3D Druck

M2

M6

M4

Materials Characterization

Biological and Bioactive Materials

Non-metallic Materials

50

30

20

https://distribution.service.intercongress.de/from.storage?image=7EqQDOTcXY0E0NJ8h-CBZwV6cGpoWOhC0FmnLgcQnKj0TSivHAxEtixcTsms5QTh0

E163-02-1 - Forschungsgruppe Polymerchemie und Technologie

E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication

E056-12 - Fachbereich ENROL DP

0000-0003-4121-2731

0000-0002-3392-5806

0000-0001-8984-5672

0000-0003-1654-462X

0000-0002-5390-0761

Tissue Engineering and Regenerative Medicine Society (TERMIS) European Congress 2025

Nationalstiftung für Forschung, Technologie und Entwicklung

Bundesministerium für Digitalisierung und Wirtschaftsstandort

20-05-2025

23-05-2025

On Site

Event for scientific audience

Freiburg

DE

Baltzaki, Maria Chrysi Izampella

Multi Track

Chemie

Chemische Verfahrenstechnik

Pharmazie, Pharmakologie, Toxikologie

1040

2040

3012

60

20

20

conference poster not in proceedings

http://purl.org/coar/resource_type/c_18co

Publications

en

none

no Fulltext

E163-02-1 - Forschungsgruppe Polymerchemie und Technologie

TU Wien, Austria

E308-50-2 - Fachgruppe Technische Assistenz

TU Wien, Austria

Ludwig Boltzmann Gesellschaft, Austria

E163-02-1 - Forschungsgruppe Polymerchemie und Technologie

0000-0003-4121-2731

0000-0001-8984-5672

0000-0003-1654-462X

0000-0002-5390-0761

E163-02 - Forschungsbereich Makromolekulare Chemie

E308-50 - Services des Instituts

E163-02 - Forschungsbereich Makromolekulare Chemie

Christian Doppler Forschungsgesells

CDL Baudis