<div class="csl-bib-body">
<div class="csl-entry">Khattab, A., Chung, H. U., Bui, M. T., & Zeck, G. M. (2026, April 10). <i>Assessment of Anti-cancer Drugs on 3D Cell Cultured Spheroids using Electrical Impedance Spectroscopy on Mesh Microelectrode Arrays</i> [Conference Presentation]. ÖGBMT-ÖGMP 2026, Innsbruck, Austria.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/227605
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dc.description.abstract
Introduction
The effects of anti-cancer drugs on 3D spheroids can be assessed using electrical impedance spectroscopy (EIS) per-formed on microelectrode arrays (MEAs). However, in planar MEAs, the solid substrate limits nutrient diffusion to the bottom of the spheroids. In addition, spheroid placement and stabilization on planar MEAs are challenging. To overcome these limitations, mesh MEAs are investigated here, allowing the culture medium to surround 3D cell-cultured spheroids and improving mass transport conditions.
Methods
The mesh MEA (MultiChannelSystem MCS GmbH, Germany) comprised of 60 electrodes of 30 µm diameter and an inter-electrode spacing of 200 µm, fabricated on a polyimide mesh. Spheroids of 250 µm size were generated from the HT-29 cell line and cultivated on mesh MEAs for 96 hours. Navitoclax (10 µM) was tested against untreated sphe-roids (n = 5 per group). EIS was performed using the impedance analyzer ISX-3 (Sciospec GmbH, Germany). The rel-ative impedance change (RIC) was calculated to assess mesh MEA performance.
Results
The mesh MEA enabled easy placement and stable holding of spheroids. Cells proliferated around the mesh structure, allowing for impedance measurements inside the spheroid and at its periphery. In both control and Navitoclax-treated groups, RIC increased up to 48 hours. After this time point, RIC values stabilized in the control group, whereas a re-duction in RIC was observed in the Navitoclax-treated spheroids. On rigid planar MEAs, a large percentage of sphe-roids moved during culture. Additionally, impedance signals were declining, potentially due to the formation of a ne-crotic core within the spheroids.
Conclusion
Mesh MEAs provide a robust platform for stable spheroid cultivation and long-term EIS measurements. Drug-induced effects can be reliably detected using mesh MEAs for 3D in vitro drug screening applications. Future work will focus on testing drug effects on spheroids derived from different cell lines on the same mesh MEA.
en
dc.description.sponsorship
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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dc.language.iso
en
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dc.subject
Mesh MEA
en
dc.subject
Electrical Impedance Spectroscopy
en
dc.subject
Tumor Spheroid
en
dc.title
Assessment of Anti-cancer Drugs on 3D Cell Cultured Spheroids using Electrical Impedance Spectroscopy on Mesh Microelectrode Arrays
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.contributor.affiliation
TU Wien, Austria
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dc.relation.grantno
895365
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dc.type.category
Conference Presentation
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tuw.project.title
Bioelektronische Plattform für personalisierte Krebstherapie auf der Grundlage von 3D-gedruckten Sphäroiden und von Patienten-abgeleiteten Mikrotumoren
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tuw.researchTopic.id
M6
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tuw.researchTopic.id
I8
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tuw.researchTopic.name
Biological and Bioactive Materials
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tuw.researchTopic.name
Sensor Systems
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tuw.researchTopic.value
50
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tuw.researchTopic.value
50
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tuw.publication.orgunit
E363 - Institut für Biomedizinische Elektronik
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tuw.author.orcid
0000-0003-3998-9883
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tuw.event.name
ÖGBMT-ÖGMP 2026
de
tuw.event.startdate
09-04-2026
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tuw.event.enddate
11-04-2026
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Innsbruck
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tuw.event.country
AT
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tuw.event.institution
ÖGBMT - ÖGMP
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tuw.event.presenter
Khattab, Ahmed
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tuw.event.track
Single Track
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wb.sciencebranch
Biologie
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wb.sciencebranch
Sonstige Medizinisch-theoretische Wissenschaften
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wb.sciencebranch
Elektrotechnik, Elektronik, Informationstechnik
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wb.sciencebranch.oefos
1060
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wb.sciencebranch.oefos
3019
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wb.sciencebranch.oefos
2020
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wb.sciencebranch.value
30
-
wb.sciencebranch.value
20
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wb.sciencebranch.value
50
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.cerifentitytype
Publications
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item.grantfulltext
none
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item.openairecristype
http://purl.org/coar/resource_type/c_18cp
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item.openairetype
conference paper not in proceedings
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crisitem.project.funder
FFG - Österr. Forschungsförderungs- gesellschaft mbH
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crisitem.project.grantno
895365
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.dept
TU Wien, Austria
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crisitem.author.dept
E363-50 - Services des Instituts
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crisitem.author.dept
E363 - Institut für Biomedizinische Elektronik
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crisitem.author.orcid
0000-0003-3998-9883
-
crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
-
crisitem.author.parentorg
E363 - Institut für Biomedizinische Elektronik
-
crisitem.author.parentorg
E350 - Fakultät für Elektrotechnik und Informationstechnik
