<div class="csl-bib-body">
<div class="csl-entry">Duong, T. P. T., Waldhör, B., Schittmayer-Schantl, M., Mikula, H., Tomin, T., & Birner-Grünberger, R. (2026). <i>"Two-Step-One-Pot” Thiol-Selective Enrichment Strategy for In-depth Redox Proteome Profiling</i> [Poster Presentation]. 18th MSBM: Fundamentals & Applications in Mass Spectrometry Summer School, Dubrovnik, Croatia.</div>
</div>
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dc.identifier.uri
http://hdl.handle.net/20.500.12708/229656
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dc.description.abstract
Cysteine oxidation is a central mechanism of redox signalling. However, the comprehensive characterization of these modifications remains limited by the sensitivity and proteome coverage of current mass spectrometry-based redox proteomics workflows. To address these challenges, we developed “Two-Step-One-Pot” cysteine enrichment strategy based on sequential isotopic labelling of reduced and oxidized cysteine residues using thiol-specific click chemistry probes. This approach enables differential cysteine labelling within a single sample, eliminating the need of separating light and heavy tagged replicates and requiring only minimal starting sample amounts. Concomitantly, systematic optimization of labelling and enrichment resulted in improved identification of cysteine-containing peptides. Application of the workflow to redox proteome profiling of cells showed increased sensitivity and improved protein sequence coverage compared to our previously published approaches. In summary, we present a novel platform for quantitative redox proteomics which enables in-depth profiling of cysteine-mediated redox regulation.
en
dc.description.sponsorship
FWF - Österr. Wissenschaftsfonds
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dc.language.iso
en
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dc.subject
redox proteomics
en
dc.subject
Cysteine oxidation
en
dc.title
"Two-Step-One-Pot” Thiol-Selective Enrichment Strategy for In-depth Redox Proteome Profiling
en
dc.type
Presentation
en
dc.type
Vortrag
de
dc.relation.grantno
COE 17-B
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dc.type.category
Poster Presentation
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tuw.project.title
Cluster of Excellence Circular Bioengineering
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tuw.researchinfrastructure
Cell Culture Core Facility (CCCF)
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tuw.researchTopic.id
X1
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tuw.researchTopic.name
Beyond TUW-research focus
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tuw.researchTopic.value
100
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tuw.publication.orgunit
E164-01-3 - Forschungsgruppe Bioanalytik
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tuw.publication.orgunit
E056-29 - Fachbereich BioDevX
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tuw.publication.orgunit
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
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tuw.publication.orgunit
E056-12 - Fachbereich ENROL DP
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tuw.author.orcid
0009-0006-4500-7347
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tuw.author.orcid
0000-0003-3249-655X
-
tuw.author.orcid
0000-0002-9218-9722
-
tuw.author.orcid
0000-0002-7071-2316
-
tuw.author.orcid
0000-0003-3950-0312
-
tuw.event.name
18th MSBM: Fundamentals & Applications in Mass Spectrometry Summer School
en
tuw.event.startdate
05-07-2026
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tuw.event.enddate
10-07-2026
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tuw.event.online
On Site
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tuw.event.type
Event for scientific audience
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tuw.event.place
Dubrovnik
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tuw.event.country
HR
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tuw.event.presenter
Duong, Thi Phuong Thanh
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tuw.event.track
Single Track
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wb.sciencebranch
Chemie
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wb.sciencebranch.oefos
1040
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wb.sciencebranch.value
100
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item.fulltext
no Fulltext
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item.languageiso639-1
en
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item.openairecristype
http://purl.org/coar/resource_type/c_18co
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item.cerifentitytype
Publications
-
item.grantfulltext
none
-
item.openairetype
conference poster not in proceedings
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crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E163-03-2 - Forschungsgruppe Molekulare Chemie und Chemische Biologie
-
crisitem.author.dept
E164-01-3 - Forschungsgruppe Bioanalytik
-
crisitem.author.dept
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.orcid
0009-0006-4500-7347
-
crisitem.author.orcid
0000-0003-3249-655X
-
crisitem.author.orcid
0000-0002-9218-9722
-
crisitem.author.orcid
0000-0002-7071-2316
-
crisitem.author.orcid
0000-0003-3950-0312
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E163-03 - Forschungsbereich Organische und Biologische Chemie
-
crisitem.author.parentorg
E164-01 - Forschungsbereich Imaging und Instrumentelle Analytische Chemie
-
crisitem.author.parentorg
E164 - Institut für Chemische Technologien und Analytik
