<div class="csl-bib-body">
<div class="csl-entry">Mandt, D., Gruber, P., Markovic, M., Tromayer, M., Rothbauer, M., Kratz, S. R. A., Faheem Ali, S., Van Hoorick, J., Holnthoner, W., Mühleder, S., Dubruel, P., Van Vlierberghe, S., Ertl, P., Liska, R., & Ovsianikov, A. (2018). Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization. <i>INTERNATIONAL JOURNAL OF BIOPRINTING</i>. https://doi.org/10.18063/IJB.v4i2.144</div>
</div>
The placenta is a transient organ, essential for development and survival of the unborn fetus. It interfaces the body of the pregnant woman with the unborn child and secures transport of endogenous and exogenous substances. Maternal and fetal blood are thereby separated at any time, by the so-called placental barrier. Current in vitro approaches fail to model this multifaceted structure, therefore research in the field of placental biology is particularly challenging. The present study aimed at establishing a novel model, simulating placental transport and its implications on development, in a versatile but reproducible way. The basal membrane was replicated using a gelatin-based material, closely mimicking the composition and properties of the natural extracellular matrix. The microstructure was produced by using a high-resolution 3D printing method – the two-photon polymerization (2PP). In order to structure gelatin by 2PP, its primary amines and carboxylic acids are modified with methacrylamides and methacrylates (GelMOD-AEMA), respectively. High-resolution structures in the range of a few micrometers were produced within the intersection of a customized microfluidic device, separating the x-shaped chamber into two isolated cell culture compartments. Human umbilical-vein endothelial cells (HUVEC) seeded on one side of this membrane simulate the fetal compartment while human choriocarcinoma cells, isolated from placental tissue (BeWo B30) mimic the maternal syncytium. This barrier model in combination with native flow profiles can be used to mimic the microenvironment of the placenta, investigating different pharmaceutical, clinical and biological scenarios. As proof-of-principle, this bioengineered placental barrier was used for the investigation of transcellular transport processes. While high molecular weight substances did not permeate, smaller molecules in the size of glucose were able to diffuse through the barrier in a time-depended manner. We envision to apply this bioengineered placental barrier for pathophysiological research, where altered nutrient transport is associated with health risks for the fetus.
en
dc.description.sponsorship
Austrian Science Funds (FWF)
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dc.description.sponsorship
European Union’s Horizon 2020
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dc.description.sponsorship
European Research Council (ERC)
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dc.language
English
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dc.language.iso
en
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dc.publisher
Whioce Publishing Pte. Ltd.
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dc.relation.ispartof
INTERNATIONAL JOURNAL OF BIOPRINTING
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dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
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dc.subject
high resolution 3D printing
en
dc.subject
placental barrier
en
dc.subject
model
en
dc.subject
microstructure
en
dc.subject
two-photon polymerization
en
dc.title
Fabrication of biomimetic placental barrier structures within a microfluidic device utilizing two-photon polymerization
en
dc.type
Article
en
dc.type
Artikel
de
dc.contributor.affiliation
TU Wien, Österreich
-
dc.contributor.affiliation
Ghent University, Belgium
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dc.contributor.affiliation
AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austria
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dc.contributor.affiliation
AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austria
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dc.contributor.affiliation
Ghent University, Belgium
-
dc.contributor.affiliation
Ghent University, Belgium
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dc.relation.grantno
I2444-N28
-
dc.relation.grantno
685817
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dc.relation.grantno
307701
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dc.rights.holder
The Author(s) 2018
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dc.type.category
Original Research Article
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true
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true
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vor
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dcterms.isPartOf.title
INTERNATIONAL JOURNAL OF BIOPRINTING
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E163 - Institut für Angewandte Synthesechemie
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tuw.publication.orgunit
E308 - Institut für Werkstoffwissenschaft und Werkstofftechnologie
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tuw.publisher.doi
10.18063/IJB.v4i2.144
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dc.identifier.eissn
2424-8002
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dc.identifier.libraryid
AC15334427
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dc.identifier.urn
urn:nbn:at:at-ubtuw:3-5363
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tuw.author.orcid
0000-0001-8984-5672
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0000-0002-9928-3631
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0000-0001-6425-9028
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0000-0001-5845-8653
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0000-0002-7625-2445
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0000-0001-7865-1936
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0000-0001-5846-0198
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CC BY-NC 4.0
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true
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with Fulltext
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http://purl.org/coar/resource_type/c_2df8fbb1
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Publications
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research article
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open
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Open Access
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en
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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crisitem.author.dept
E308 - Institut für Werkstoffwissenschaft und Werkstofftechnologie
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E308-50-2 - Fachgruppe Technische Assistenz
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crisitem.author.dept
TU Wien, Österreich
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E163-03-1 - Forschungsgruppe Cell Chip
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E163-03-1 - Forschungsgruppe Cell Chip
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crisitem.author.dept
AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austria
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crisitem.author.dept
AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austria
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crisitem.author.dept
E610 - Vizerektorat Forschung und Innovation
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crisitem.author.dept
E163-02-1 - Forschungsgruppe Polymerchemie und Technologie
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crisitem.author.dept
E308-02-3 - Forschungsgruppe 3D Printing and Biofabrication
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0000-0001-8984-5672
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E308-02 - Forschungsbereich Polymer- und Verbundwerkstoffe
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E300 - Fakultät für Maschinenwesen und Betriebswissenschaften
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E308-50 - Services des Instituts
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E163-03 - Forschungsbereich Organische und Biologische Chemie
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E163-03 - Forschungsbereich Organische und Biologische Chemie