<div class="csl-bib-body">
<div class="csl-entry">Ehgartner, D., Herwig, C., & Fricke, J. (2017). Morphological analysis of the filamentous fungus Penicillium chrysogenum using flow cytometry—the fast alternative to microscopic image analysis. <i>Applied Microbiology and Biotechnology</i>, <i>101</i>(20), 7675–7688. https://doi.org/10.1007/s00253-017-8475-2</div>
</div>
An important parameter in filamentous bioreactor cultivations is the morphology of the fungi, due to its interlink to productivity and its dependency on process conditions. Filamentous fungi show a large variety of morphological forms in submerged cultures. These range from dispersed hyphae, to interwoven mycelial aggregates, to denser hyphal aggregates, the so-called pellets. Depending on the objective function of the bioprocess, different characteristics of the morphology are favorable and need to be quantified accurately. The most common method to quantitatively characterize morphology is image analysis based on microscopy. This method is work intensive and time consuming. Therefore, we developed a faster, at-line applicable, alternative method based on flow cytometry. Within this contribution, this novel method is compared to microscopy for a penicillin production process. Both methods yielded in comparable distinction of morphological sub-populations and described their morphology in more detail. In addition to the appropriate quantification of size parameters and the description of the hyphal region around pellets, the flow cytometry method even revealed a novel compactness parameter for fungal pellets which is not accessible via light microscopy. Hence, the here presented flow cytometry method for morphological analysis is a fast and reliable alternative to common tools with some new insights in the pellet morphology, enabling at-line use in production environments.
en
dc.language
English
-
dc.language.iso
en
-
dc.publisher
Springer
-
dc.relation.ispartof
Applied Microbiology and Biotechnology
-
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
-
dc.subject
Filamentous fungi
en
dc.subject
Flow cytometry
en
dc.subject
Morphology
en
dc.subject
Pellets
en
dc.subject
Microscopy
en
dc.subject
Image analysis
en
dc.subject
Hyphae
en
dc.title
Morphological analysis of the filamentous fungus Penicillium chrysogenum using flow cytometry—the fast alternative to microscopic image analysis
en
dc.type
Article
en
dc.type
Artikel
de
dc.rights.license
Creative Commons Namensnennung 4.0 International
de
dc.rights.license
Creative Commons Attribution 4.0 International
en
dc.description.startpage
7675
-
dc.description.endpage
7688
-
dc.rights.holder
The Author(s) 2017
-
dc.type.category
Original Research Article
-
tuw.container.volume
101
-
tuw.container.issue
20
-
tuw.journal.peerreviewed
true
-
tuw.peerreviewed
true
-
tuw.version
vor
-
dcterms.isPartOf.title
Applied Microbiology and Biotechnology
-
tuw.publication.orgunit
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften
-
tuw.publisher.doi
10.1007/s00253-017-8475-2
-
dc.date.onlinefirst
2017-09-14
-
dc.identifier.eissn
1432-0614
-
dc.identifier.libraryid
AC15189806
-
dc.identifier.urn
urn:nbn:at:at-ubtuw:3-4371
-
tuw.author.orcid
0000-0003-2314-1458
-
dc.rights.identifier
CC BY 4.0
de
dc.rights.identifier
CC BY 4.0
en
wb.sci
true
-
item.fulltext
with Fulltext
-
item.cerifentitytype
Publications
-
item.openairecristype
http://purl.org/coar/resource_type/c_2df8fbb1
-
item.languageiso639-1
en
-
item.openaccessfulltext
Open Access
-
item.openairetype
research article
-
item.grantfulltext
open
-
crisitem.author.dept
E166 - Institut für Verfahrenstechnik, Umwelttechnik und technische Biowissenschaften