Saghafi, S., Becker, K., Gori, F., Foroughipour, M., Bollwein, C., Foroughipour, S. M., Steiger, K., Weichert, W., & Dodt, H. U. (2022). Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order. Journal of Biophotonics, 15(6), Article e202100342. https://doi.org/10.1002/jbio.202100342
Lasers are fundamental tools in research and development. The shape of an incident laser beam directly affects the results, when it propagates through complex structured meso-aspheric optical elements. In conic-based systems utilizing elements such as axicons, the impact of secondary lobes is mostly overlooked, although the intensity distributions at the central spot and the side-lobes directly affect the beam properties. We investigate the interaction of two axicons (160° and 170°) with incident beams approximated by Gaussian, high-order Flattened-Gaussian, and low-order Flattened-Gaussian functions. We demonstrate that replacing an incident Gaussian beam with a low-order Flattened-Gaussian beam reduces the secondary lobes and significantly improves the uniformity of the intensity profile. We practically applied this effect in engineering a conic-aspheric-based static light-sheet microscope producing markedly improved results.
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Project (external):
Austrian Science Fund (FWF) WWTF
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Project ID:
P 31263-B26 CS18-019
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Research Areas:
Biological and Bioactive Materials: 50% Modeling and Simulation: 50%