Kalaus, H., Obleser, K., Scheibelreiter, V., Seidl, B., Kozich, M., Stanetty, C., & Mihovilovic, M. (2022, November 9). Facile Quantification of Aldehyde Starch in a Photometric Assay based on 2-Aminobenzamide Oxime [Poster Presentation]. 18th International Conference on Polysaccharides-Glycoscience 2022, Prag, Czechia. http://hdl.handle.net/20.500.12708/136102
18th International Conference on Polysaccharides-Glycoscience 2022
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Event date:
9-Nov-2022 - 11-Nov-2022
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Event place:
Prag, Czechia
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Keywords:
Polysaccharides; ABAO; Quantification
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Abstract:
The oxidation of starch to aldehyde starch opens up a wide range of further functionalization, e.g., via cross-linking. In investigating this oxidation, we found that classical analytical methods rely on time-consuming and work-intensive titration protocols. To accelerate the screening process we implemented a photometric assay based on the selective adduct-formation of 2 aminobenzamide oxime (ABAO) and aldehydes. The UV-active adduct can easily be quantified in a platereader, allowing for parallel processing of multiple samples with a readout in a fraction of the time and in consistency of conventional methods.
We thank Lisa Riedlsperger for their technical support. The providence Lower Austria (BioSet, K3-F-603/003-2018) is gratefully acknowledged for financial support.
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Project title:
Mechanistische Untersuchungen zur enzymatischen Oxidation von Stärke und Lignin als Basis für neue biobasierter Klebstoffe: K3-F-603/003-2018 (Land NIEDERÖSTERREICH p.A. Amt der NÖ Landesregierung)