Kopp, J., & Spadiut, O. (2021). A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3). In K. Turksen (Ed.), Bioreactors in Stem Cell Biology (Vol. 2436, pp. 223–240). https://doi.org/10.1007/7651_2021_424
Continuous processing allows to maximize space-time yields and is implemented in many industrial branches. However, in manufacturing of value added compounds produced with microbial hosts, continuous processing is not state-of-the-art yet. This is because fluctuating productivity causes unwanted process deviations. Cascaded continuous bioprocessing, unlike conventional continuous process modes, was found to result in stable productivity. This manuscript serves as a guideline how to set up a cascaded continuous cultivation with Escherichia coli BL21 DE(3).
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Research Areas:
Biological and Bioactive Materials: 50% Sustainable Production and Technologies: 50%
