Sanchez-Illiana, A., Mayr, F., Cuesta-Garcia, D., Pineiro-Ramos, J. D., Cantarero, A., de la Guardia, M., Vento, M., Lendl, B., Quintas, G., & Kuligowski, J. (2018). On-Capillary Surface-Enhanced Raman Spectroscopy: Determination of Glutathione in Whole Blood Microsamples. Analytical Chemistry, 90(15), 9093–9100. https://doi.org/10.1021/acs.analchem.8b01492
Oxidative stress monitoring in the neonatal period supports
early outcome prediction and treatment. Glutathione (GSH) is
the most abundant antioxidant in most cells and tissues,
including whole blood, and its usefulness as a biomarker has
been known for decades. To date, the available methods for
GSH detn. require laborious sample processing and the use of
sophisticated lab. equipment. To the best of the authors'
knowledge, no tools suitable for point-of-care (POC) sensing
have been reported. Surface-enhanced Raman spectroscopy
(SERS), performed in a microvolume capillary measurement
cell, is proposed in this study as a robust approach for the
quantification of GSH in human whole blood samples. The use
of a silver colloid allowed a highly selective signal
enhancement for GSH providing anal. enhancement factors of
3 to 4 orders of magnitude. A highly accurate detn. of GSH in
whole blood samples with recoveries ranging from 99 to 107%
and relative std. deviations less than or equal to 18% were
achieved by signal normalization with the intensity of an
isotopically labeled internal std. GSH concns. were retrieved
within 4 min using small-vol. blood samples (2 mL). The
developed procedure was applied to the anal. of blood of 20
healthy adults and 36 newborns, obtaining comparable results
between literature and those found by SERS and a ref. method.
The characteristics of this novel tool are suitable for its
implementation in a portable optical sensor device enabling
POC testing of oxidative stress levels in newborns.
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Research Areas:
Biological and Bioactive Materials: 20% Materials Characterization: 60% Surfaces and Interfaces: 20%
