Record link: 
http://hdl.handle.net/20.500.12708/152493
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Title: 
Dead or alive: a novel PAT tool for the extremophile Sulfolobus acidocaldarius
en
Citation: 
Rastädter, K., Tramontano, A., Wurm, D. J., Spadiut, O., & Quehenberger, J. (2022, March 29). Dead or alive: a novel PAT tool for the extremophile Sulfolobus acidocaldarius [Conference Presentation]. 7th BioProScale Symposium 2022, Germany. http://hdl.handle.net/20.500.12708/152493
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Publication Type: 
Presentation - Conference Presentation
en
Language: 
English
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Authors: 
Rastädter, Kerstin 
Tramontano, Andrea 
Wurm, David Johannes 
Spadiut, Oliver 
Quehenberger, Julian 
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Organisational Unit: 
E166-04-2 - Forschungsgruppe Integrierte Bioprozessentwicklung 
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Date (published): 
29-Mar-2022
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Event name: 
7th BioProScale Symposium 2022 
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Event date: 
28-Mar-2022 - 5-Apr-2022
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Event place: 
Germany
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Keywords: 
Sulfolobus acidocaldarius; Flow cytometry; Live/dead staining
en
Project (external): 
FWF
ÖAW - L'ORÉAL fellowship
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Project ID: 
I 4508
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Link (external): 
https://biotechnologie.ifgb.de/bioproscale2022
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Additional information: 
Viability of Sulfolobus acidocaldarius, an extremophilic Archaeon thriving at 75 °C and pH 3.0, is still determined via laborious, time- and material-intensive plating assays. Sulfolobus harbor great potential as still untapped production hosts and biocatalysts in biotechnological applications, nevertheless no industrial processes have been developed yet. During development and scaling of industrial bioprocesses it is crucial to monitor the impact of process parameters on the cultivated organism. Flow cytometry (FCM) combines the ability to analyze single-cell properties in a cell population with viability assessment via the use of fluorescent-active dyes. In this study, commercially available fluorescent- active dyes applicable in S. acidocaldarius were identified. The dyes, Fluorescein diacetate and Concanvalin A conjugated with rhodamin, are suitable for viability determination via FCM. For showing the applicability of the developed at-line PAT tool for bioprocess monitoring a chemostat cultivation at 75°C and pH 3.0 was conducted. Over 800 hours, this novel PAT tool successfully allowed monitoring of viability. In my talk, I will introduce our exciting PAT tool paving the way for an untapped Archaeon into industrial application.
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Research Areas: 
Beyond TUW-research foci: 100%
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Science Branch: 
2040 - Chemische Verfahrenstechnik: 10%
1060 - Biologie: 30%
2090 - Industrielle Biotechnologie: 60%
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