Title: Linking Adhesion Force to Area in Molecular Contact measured by FRET spectroscopy
Authors: Simoes, Monica 
Urstöger, Georg 
Schennach, Robert 
Hirn, Ulrich 
Keywords: adhesion force; FRET methods; fluorescence dyes; area in molecular contact
Issue Date: Dec-2020
Book Title: Proceedings of the 16th Minisymposium Verfahrenstechnik and 7th Partikelforum (TU Wien, Sept. 21/22, 2020) 
Abstract: 
Adhesion depends on the area in molecular contact (AMC). Though, is extremely difficult to measure AMC, mostly because areas that appear to be in full contact, show no AMC, when observed at higher magnification. Here, we show how AMC can be measured using Förster resonance energy transfer (FRET) spectroscopy, a technique capable of measuring the nanometric distance between interactive surfaces. FRET uses the non-radiative energy transferred between fluorescence molecules (donor and acceptor) to study their exact molecular distance. If the molecules are close enough to each other (molecular contact), a FRET signal can be detected. Through a FRET signal, it is possible to calculate the energy transfer efficiency which indicates the degree of molecular contact between the surfaces. For the experiments, poly(2-Hydroxyethyl methacrylate) thin films labelled with the fluorescence molecules, 7-(diethylamino)coumarin-3-carbohydrazide (donor) and fluorescein-5-thiosemicarbazide (acceptor), were produced at the same dye(s) molar concentration and bonded using different pressure loads. Our results reveal that AMC increases with the pressure applied in the bonded thin films, resulting in higher FRET signal and adhesion force.  Hence, in this work, we implement an experimental measuring method for AMC and validate the relationship between AMC (measured with FRET spectroscopy) and adhesion (force energy) within polymeric thin films.
URI: http://hdl.handle.net/20.500.12708/16665
http://dx.doi.org/10.34726/608
DOI: 10.34726/608
Organisation: E166-02-2 - Forschungsgruppe Fluiddynamische Simulation (CFD) 
License: CC BY 4.0 CC BY 4.0
Publication Type: Inproceedings
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Appears in Collections:Conference Paper

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