Kogler, D. (2023). Approach to validation of Biolayer Interferometry for characterization of recombinant adeno-associated viral vectors : Regarding titer and degree of filling based on ICH Q2 (R1) [Diploma Thesis, Technische Universität Wien]. reposiTUm. https://doi.org/10.34726/hss.2023.95564
E164 - Institut für Chemische Technologien und Analytik
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Date (published):
2023
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Number of Pages:
109
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Keywords:
Virus; Capsid Analytik
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Virus; Capsid Analysis
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Abstract:
Goal of present master thesis is to assess BLI as possible measuring technique for both titer and filling degree, facilitating a novel method for capsid particle conglomerates derived from biotechnologically relevant (early-stage) harvest samples with accuracy lower than 20%. BLI is based on white light interferometry, which enables time-resolved monitoring of specific rAAV layer thickness accretion, hence allowing determination of titer and possibly filling degree.
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