Kopp, J., & Spadiut, O. (2023). Inclusion Bodies: Status Quo and Perspectives. In J. Kopp & O. Spadiut (Eds.), Inclusion Bodies : Methods and Protocols (Vol. 2617, pp. 1–13). Humana. https://doi.org/10.1007/978-1-0716-2930-7_1
Escherichia coli; E. coli; IB-based products; Inclusion bodies; Inclusion body processing; Recombinant protein production; Inclusion Bodies; Recombinant Proteins; inclusion body refolding; inclusion body challenges; inclusion body quality
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Abstract:
Multiple E. coli cultivations, producing recombinant proteins, lead to the formation of inclusion bodies (IBs). IBs historically were considered as nondesired by-products, due to their time- and cost-intensive purification. Nowadays, many obstacles in IB processing can be overcome. As a consequence, several industrial processes with E. coli favor IB formation over soluble production options due to the high space time yields obtained. Within this chapter, we discuss the state-of-the art biopharmaceutical IB process, review its challenges, highlight the recent developments and perspectives, and also propose alternative solutions, compared to the state-of-the art processing.
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Research Areas:
Biological and Bioactive Materials: 50% Efficient Utilisation of Material Resources: 50%
